AI Article Synopsis
- Preparative chromatographic enantioseparation is the top method for isolating pure enantiomers during the early development of new drugs.
- Supercritical fluid chromatography was chosen for this process due to its advantages over traditional liquid chromatography.
- A total of 10 mg of each enantiomer of a new anti-inflammatory compound was successfully isolated, although some peak distortion occurred during testing, likely caused by the method of injection used.
Article Abstract
Preparative chromatographic enantioseparation is now the preferred technique for obtaining milligram quantities of pure enantiomers in the initial phase of development of a therapeutic compound. Supercritical fluid chromatography offers several advantages over liquid chromatography and was therefore selected for the preparative enantioseparation of a new potential anti-inflammatory molecule. Approximately 10 mg of each of the two enantiomers was successfully prepared using a Chiralpak AD-H (tris-3,5-dimethylphenylcarbamate of amylose) polysaccharide-based stationary phase with 40% of ethanol as a co-solvent, using a stacked injection mode. A peak distortion was observed during volume overloading, which may be due to the mixed-stream injection method.
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| http://dx.doi.org/10.1002/bmc.6030 | DOI Listing |
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