Background: Candida albicans is a pathogenic commensal fungus. Trichophyton mentagrophytes and Trichophyton rubrum are the leading pathogens of dermatophysis. Rumex japonicus Houtt. has a miraculous effect on the treatment of tinea skin disease, but its mechanism has not been clarified.
Purpose: This paper investigated the anti-fungal ingredients of the leaves of Rumex japonicus Houtt. (RJH-L) and the mechanism of the anti-fungal (Trichophyton mentagrophytes, Trichophyton rubrum and Candida albicans).
Method: First, the chemical composition analysis of RJH-L was conducted by acid extraction and alcohol precipitation, high performance liquid chromatography (HPLC) and nuclear magnetic resonance spectroscopy (NMR); in vitro anti-fungal experiments were carried out, including the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) spore germination rate, germ tube production rate, nucleic acid and protein leakage rate, biofilm structure, PCR, etc., to study the mechanism of action of RJH-L anti-fungal and anti-biofilm activity.
Result: Seven monomer compounds were obtained: anthraquinones (rhein, emodin and aloe-emodin); polyphenols (ferulic acid, p-coumaric acid), and flavonoids (rutin and quercetin). The results of in vitro anti-fungal experiments showed that the extracts of RJH-L had strong inhibitory effect on both fungi (MIC: 1.96 µg/mL-62.50 µg/mL), of which emodin had the strongest effect on Trichophyton mentagrophytes; and rhein had the strongest effect on Candida albicans and Trichophyton rubrum. The above active components can inhibit the germination of fungal spores and germ tube, change cell membrane permeability, prevent hyphal growth, destroy the biofilm structure, and down-regulate the expression of agglutinin-like sequencefamily1 of biofilm growth.
Conclusion: This study shows that RJH-L are rich in polyphenols, flavonoids, and anthraquinones, and play a fungicidal role.
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http://dx.doi.org/10.1016/j.mycmed.2024.101513 | DOI Listing |
J Mycol Med
December 2024
Department of School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, 130117 Jilin, PR China. Electronic address:
Background: Candida albicans is a pathogenic commensal fungus. Trichophyton mentagrophytes and Trichophyton rubrum are the leading pathogens of dermatophysis. Rumex japonicus Houtt.
View Article and Find Full Text PDFJ Nat Med
September 2024
Department of School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, 130117, Jilin, People's Republic of China.
Front Pharmacol
June 2024
Key Laboratory of Specialty Agri-product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Science, China Jiliang University, Hangzhou, China.
Rhubarb is a frequently used and beneficial traditional Chinese medicine. Wild resources of these plants are constantly being depleted, meaning that rhubarb products have been subjected to an unparalleled level of adulteration. Consequentially, reliable technology is urgently required to verify the authenticity of rhubarb raw materials and commercial botanical drugs.
View Article and Find Full Text PDFChemosphere
June 2024
School of Water Conservancy and Transportation, Zhengzhou University, Henan, 450001, China. Electronic address:
Water eutrophication caused by nitrogen pollution is an urgent global issue that requires attention. The Qingyi River is a typical micro-polluted river in China. In this study, we took this river as the research object to investigate the nitrogen pollution purification capacity of a herbaceous plant, Rumex japonicus Houtt.
View Article and Find Full Text PDFJ AOAC Int
October 2022
Changchun University of Chinese Medicine, School of Pharmaceutical Sciences, Changchun 130117, China.
Background: Rumex japonicus Houtt (R. japonicus) is used mainly to treat various skin diseases in Southeast Asia. However, there are few studies on its quality evaluation methods and antifungal activity.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!