AI Article Synopsis
- Raman spectroscopy was utilized to assess the effects of cytosine arabinoside (ara-C) on live neurons, which is important for selectively culturing neurons while excluding non-neuronal cells.
- The study involved comparing Raman spectra from neurons exposed to different concentrations of ara-C (0, 5, and 25 µM) to determine how these levels impacted neurodevelopment.
- Findings indicated that exposure to 5 µM ara-C did not significantly change neuronal characteristics, whereas 25 µM caused profound developmental alterations, emphasizing the effectiveness of Raman spectroscopy to detect minor physiological changes in neurons due to chemical exposure.
Article Abstract
Raman spectroscopy is used to monitor the development of live neurons exposed to cytosine arabinoside (ara-C). Ara-C is widely used to culture neurons and exclude non-neuronal cells. In this study, Raman spectra obtained from neurons exposed to ara-C were plotted using an analytical model of neuronal development to evaluate the impact of ara-C on neuronal development. After two days of culturing, neurons were exposed to ara-C for 24 h at final concentrations of 0 (control), 5, and 25 μM. Principal component analysis (PCA) was performed to build an analytical model for evaluating neurodevelopmental disorders caused by ara-C treatment. We projected the Raman spectra obtained from ara-C-treated cells onto the control group dataset. The distribution of PC1 scores for neurons exposed to ara-C at a final concentration of 5 μM was not significantly different from that of the control group. In contrast, under a final concentration of 25 μM, the data population at 10 and 15 days of culturing overlapped significantly with that of neurons at 4 days of normal culturing. These results suggest that Raman spectroscopy can detect very small physiological alterations in the neurons even after a short-term exposure (24 h) of ara-C. Our analytical method has high potential to evaluate the developmental stages for living neurons under exposure to chemicals.
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| http://dx.doi.org/10.1177/00037028241289147 | DOI Listing |
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