Gas chromatography (GC) was used to determine the fatty acid (FA) compositions of total lipid, phospholipid (PL), phospholipid subclass (phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE)), and TAG (triacylglycerol) fractions in male Chondrostoma regium. Percentages of myristic acid (14:0), palmitoleic acid (16:1n-7), oleic acid (18:1n-9), monounsaturated fatty acid (ΣMUFA), linoleic acid (18:2n-6) and linolenic acid (18:3n-3) were found to be higher in TAG than the values determined in PL classes. Palmitic acid (16:0), eicosapentaenoic acid (EPA, 20:5n-3), docosahexaenoic acid (DHA, 20:6n-3) in the PC fraction; 16:0, 20:5n-3, 22:6n-3 in PE; 16:0, stearic acid (18:0), arachidonic acid (AA, 20:4n-6), 20:5n-3, 22:6n-3 in PI; and 16:0, 18:0, oleic acid (18:1n- 9), 22:6n-3 in PS were found to be dominant. In total lipid, the PUFA/SFA ratio was 1.37-1.83; atherogenicity index (AI) was 0.34-0.47 and thrombogenicity index (TI) was found in the range of 0.18-0.22. The n-3/n-6 ratio, which is an important index for nutritional content, was found to be between 5.15 and 11.07. It was discovered that the FA compositions of male C. regium were affected by the reproductive period and season. These findings suggest that fish oil might be a beneficial dietary source for preserving human health.

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