AI Article Synopsis
- Solid-state nanochannel biosensors are effective for detecting microRNA due to their sensitivity but have limited dynamic ranges that affect their performance.
- The authors propose using tunable triblock DNA probes to enhance these biosensors, allowing for customizable adjustments to their dynamic range and improving their binding affinities for target miRNA.
- By utilizing these advanced probes, the biosensors achieve an impressive dynamic range increase, from an initial 81-fold to 10,900-fold, which greatly expands their potential for use in various biomedical and clinical applications.
Article Abstract
Solid-state nanochannel biosensors are extensively utilized for microRNA (miRNA) detection owing to their high sensitivity and rapid response. However, conventional nanochannel biosensors face limitations in their fixed dynamic range, restricting their versatility and efficacy. Herein, we introduce tunable triblock DNA probes with varying affinities for target miRNA to engineer solid-state nanochannel biosensors capable of customizable dynamic range adjustment. The triblock DNA architecture comprises a poly-adenine (polyA) block for adjustable surface density anchoring, alongside stem and loop blocks for modulating structural stability. Through systematic manipulation of these blocks, we demonstrate the ability to achieve diverse target binding affinities and detection limits, achieving an initial 81-fold dynamic range. By combining probes with various affinities, we extend this dynamic range significantly to 10,900-fold. Furthermore, by implementing a sequestration mechanism, the effective dynamic range of the nanochannel biosensor is narrowed to only a 3-fold span of target concentrations. The customizable dynamic range of these advanced nanochannel biosensors makes them highly promising for a broad spectrum of biomedical and clinical applications.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/anie.202417280 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The development of social attention in orangutans: Comparing peering behavior in wild and zoo-housed individuals.
iScience
January 2025
Development and Evolution of Cognition Research Group, Max Planck Institute of Animal Behavior, Bücklestraße 5 78467 Konstanz, Germany.
Social learning plays an essential role in all cultural processes, but the factors underlying its evolution remain poorly understood. To understand how socio-ecological conditions affect social learning, we compared peering behavior (i.e.
View Article and Find Full Text PDFImpact of ultrasound settings on lung vertical artefacts: an observational study in mechanically ventilated patients.
ERJ Open Res
January 2025
Critical Care Department, Hospital Garcia de Orta EPE, Almada, Portugal.
Introduction: The number of vertical artefacts (VAs) in lung ultrasound (LUS) impacts patients' clinical management. This study aimed to demonstrate the influence of ultrasound settings on the number of VAs in patients under invasive mechanical ventilation (IMV).
Methods: Patients under IMV were recruited for LUS, including three breathing cycles with a motionless curvilinear probe on the thoracic region with the most VAs.
Temperature-Dependent Formation of Carbon Nanodomains in Silicon Oxycarbide Glass-A Reactive Force Field MD Study.
J Phys Chem C Nanomater Interfaces
January 2025
Institute of General, Inorganic and Theoretical Chemistry Center for Chemistry and Biomedicine, University of Innsbruck, Innrain 80-82, A-6020 Innsbruck, Austria.
Novel anode materials for lithium-ion batteries (LIBs) are constantly being explored to further improve battery performance. In this work, ReaxFF molecular dynamics (MD) simulations are performed to model the early stages in the synthesis of nanostructured silicon carbide (SiC), which is one such promising material. The focus lies on its precursor, silicon oxycarbide glass of composition (SiOC) (17 mol% Si, 28 mol% O, and 54 mol% C), in the following referred to as SiOC.
View Article and Find Full Text PDFExploring the capabilities of 2-alkynyl aryl/benzyl azides: synthesis approaches for indoles, quinolines, and their derivatives transition metal catalysis.
RSC Adv
January 2025
The Organic Chemistry Research Laboratory (OCRL), Department of Chemistry, Faculty of Science, University of Zanjan Zanjan 45371-38791 Iran
In recent research, quinoline and indole structures have gained recognition for their significant clinical relevance and effectiveness. These compounds are known for their wide-ranging pharmacological effects, which include anticancer, antibacterial, antifungal, antiviral, and anti-inflammatory properties. Researchers have successfully implemented a variety of innovative synthetic strategies, leading to the creation of numerous compounds that display fascinating biological activities in diverse fields.
View Article and Find Full Text PDFContinuous reference intervals for plasma cystatin C and creatinine in Vietnamese children.
EJIFCC
December 2024
Department of Laboratory Medicine, National University Hospital, Singapore.
Background: Serum (plasma) creatinine and cystatin C are widely used in pediatric clinical practice to assess glomerular filtration rate. Both markers have limitations due to the low index of individuality, which affects the clinical sensitivity of population-based reference intervals, especially when wide age ranges are considered. This study aimed to establish age-related reference intervals for plasma cystatin C and creatinine in Vietnamese children.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!