AI Article Synopsis
- - G-quadruplexes (G4s) in RNA are becoming important targets for cancer and disease therapies, with GG motifs being more common than GGG motifs in RNA sequences.
- - Recent findings indicate that DNA cannot make stable two-tetrad G4 structures, raising questions about the stability of RNA GG motifs and their potential regulatory functions.
- - This study reveals that RNA GG motifs typically don't form stable intramolecular G4s by themselves; instead, they need to dimerize to create stable structures, which may play a role in RNA aggregation during cellular stress.
Article Abstract
G-quadruplexes (G4s) formed within RNA are emerging as promising targets for therapeutic intervention in cancer, neurodegenerative disorders and infectious diseases. Sequences containing a succession of short GG blocks, or uneven G-tract lengths unable to form three-tetrad G4s (GG motifs), are overwhelmingly more frequent than canonical motifs involving multiple GGG blocks. We recently showed that DNA is not able to form stable two-tetrad intramolecular parallel G4s. Whether RNA GG motifs can form intramolecular G4s under physiological conditions and play regulatory roles remains a burning question. In this study, we performed a systematic analysis and experimental evaluation of a number of biologically important RNA regions involving RNA GG motifs. We show that most of these motifs do not form stable intramolecular G4s but need to dimerize to form stable G4 structures. The strong tendency of RNA GG motif G4s to associate may participate in RNA-based aggregation under conditions of cellular stress.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11602125 | PMC |
| http://dx.doi.org/10.1093/nar/gkae927 | DOI Listing |
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