Reviews of bio-orthogonal probes in bioscience by stimulated Raman scattering microscopy.

Spectrochim Acta A Mol Biomol Spectrosc

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, 830017, China; Institute of Materia Medica, Xinjiang University, Urumqi, 830017, China. Electronic address:

Published: October 2023

AI Article Synopsis

  • Stimulated Raman scattering (SRS) microscopy is a powerful imaging method that uses molecular vibrational bonds to visualize chemical content with high sensitivity and specificity.
  • * The review discusses recent advancements in SRS combined with bio-orthogonal Raman tags in bioscience, detailing how these tags work and their various applications.
  • * The authors highlight current challenges in the field and propose future research directions, emphasizing the potential of SRS technology to drive major discoveries in biology.*

Article Abstract

Stimulated Raman scattering (SRS) microscopy, is a nonlinear optical imaging method for visualizing chemical content based on molecular vibrational bonds, with high sensitivity, resolution, speed, and specificity. In the current review, we provided a comprehensive and critical review of the most recent developments in the field of SRS in combination with bio-orthogonal Raman tags or labels in bioscience. Firstly, we introduced the fundamentals of SRS microscopy and the theory principle of bio-orthogonal Raman tags. In particular, present the applications of each kind of bio-orthogonal Raman tags, including heavy water (DO), stable isotope probes (SIP), and triple-bonds tags. And shared our vision for the remaining challenges, research needs, and potential future breakthroughs for SRS technology lastly. We envision that the advanced SRS imaging and analysis will be a major force in future biological discovery.

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Source
http://dx.doi.org/10.1016/j.saa.2023.123545DOI Listing

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