Research in the areas of organelle dynamics, cytoskeletal interactions, membrane protrusions, and cell motility relies heavily on live-cell imaging. These structures continuously move about in complex patterns and imaging them live at sufficient temporal resolutions as well as for durations long enough to extract significant number of events is an absolute necessity. Capturing most of the sub-cellular dynamics in whole cell volumes was beyond reach due to the lack of balance between reduced photo-toxicity, time resolution, and the required spatial resolution in dominant imaging modalities like point scanning confocal and spinning disc confocal microscopy. In the last few years, a plethora of light-sheet geometries have emerged, pushing the limits of measurements. In this review, we will focus on a subset of light-sheet modalities that are most suited to studying live, sub-cellular dynamics in whole-cell volumes.
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