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Synthesis, characterization and MAFLD prevention potential of Ganoderma lucidum spore polysaccharide-stabilized selenium nanoparticles. | LitMetric

Synthesis, characterization and MAFLD prevention potential of Ganoderma lucidum spore polysaccharide-stabilized selenium nanoparticles.

Int J Biol Macromol

Horticulture College, Hunan Agricultural University, Changsha, Hunan, PR China; State Key Laboratory of Subhealth Intervention Technology, Changsha, Hunan, PR China; Hunan Provincial Engineering Research Center of Medical Nutrition Intervention Technology for Metabolic Diseases, Hunan Agricultural University, Changsha, Hunan, PR China; Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha, Hunan, PR China. Electronic address:

Published: December 2024

AI Article Synopsis

  • The study focused on the stabilization of selenium nanoparticles (SeNPs) using Ganoderma lucidum spore polysaccharide (GLP), which showed improved stability and therapeutic potential.
  • GLP-SeNPs, created in a specific ratio, demonstrated beneficial effects in reducing oxidative stress and lipid accumulation in liver cells, contributing to the prevention of metabolic-associated fatty liver disease (MAFLD).
  • The mechanism of action involves the regulation of various pathways, including iron metabolism and antioxidant defense, suggesting GLP-SeNPs as a potential dietary supplement for MAFLD prevention.

Article Abstract

The unstability of selenium nanoparticles (SeNPs) results in decreased activity which limits its therapeutic potential. In this study, we utilized Ganoderma lucidum spore polysaccharide (GLP, Mw = 983.96 kDa) as a novel stabilizer to synthesize GLP-SeNPs. GLP-SeNPs (Se/GLP = 1/3) with an average diameter of 149 nm were successfully prepared and it was stable for at least 30 days at 4 °C. It exhibited an orange-red color, zero valence state, amorphous structure, selenium uniform distribution, a zeta potential of -29.73 mV, selenium content of 16.04 %. GLP-SeNPs pretreatment decreased lipid accumulation, reduced ROS content and enhanced SOD and CAT activity in HepG2 cells. Fe and MDA contents were decreased, while GPX4 and GSH activities were increased. All these ameliorated effects could be abolished by NRF2 antagonist ML385. The expression of anti-oxidant genes and iron exporter was up-regulated, while that of pro-oxidant and lipid biosynthesis gene was down-regulated. The GPX4 activity could be reduced by ML385 addition. In conclusion, GLP-SeNPs was successfully constructed at the ratio of 1/3 (Se/GLP). It prevents MAFLD by targeting ferroptosis, including lowering iron overload, inhibiting lipid accumulation and attenuating oxidative stress. The improvement was conducted via activating SLC40A1-mediated iron pathway, ACSL4-mediated lipid metabolism and NRF2-mediated GSH-GPX4 pathway. Therefore, GLP-SeNPs can be used as potential selenium nutritional supplements or adjuvants for MAFLD prevention.

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Source
http://dx.doi.org/10.1016/j.ijbiomac.2024.136962DOI Listing

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