Fabrication of porcine acellular dermal matrix and oxidized hyaluronic acid conductive composite hydrogels for accelerating skin wound healing under electrical stimulation.

Electrical stimulation (ES) of skin wounds can promote cell proliferation, protein synthesis, inflammatory response, and improve neurological function. In this study, the dynamical cross-linked conductive hydrogels have been developed by integration of poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) into porcine acellular dermal matrix (PADM) and oxidized hyaluronic acid (OHA) using dopamine-Fe complex (DA@Fe) as cross-linking agent. The as-obtained conductive composite hydrogels have the characteristics of rapid gelation, excellent deformation ability, high water absorption, and suitable degradation rate. Four-point probes resistivity measurement system has been used to test the electrical properties of the as-obtained hydrogels, and showing their conductivity at 0.207-0.322 S/m. In addition, the developed dynamical cross-linked conductive hydrogels exhibit excellent biocompatibility, thereby inducing cells proliferation and migration. In vivo results show that the resultant composite hydrogel can accelerate wound healing with combination of electrical stimulation (ES) by promoting expression of vascular factor (CD31) and decreasing the expression of tumor necrosis factor-α (TNF-α).