AI Article Synopsis
- Lipid accumulation in macrophages is linked to their role in promoting tumor growth, emphasizing their importance in cancer biology.
- The text outlines a workflow for studying how macrophages interact with lipid-laden tumor cells, detailing methods for identifying and analyzing these macrophages in mouse tumors.
- This research aims to reveal the origins of lipids that contribute to the formation of lipid-laden macrophages and their potential to enhance cancer malignancy across different types of cancer.
Article Abstract
Lipid accumulation has recently emerged as a key feature underlying the pro-tumorigenic role of macrophages. Here, we present a workflow to study macrophage lipid crosstalk with tumor cells. We describe steps for the identification, purification, and multi-omics characterization of lipid-laden macrophages (LLMs) from murine tumors and outline protocols to assess the functional significance of LLMs in cancer malignancy. This approach has the potential to uncover the source of lipids that drives LLM formation and its pro-tumorigenic potential in multiple cancer types. For complete details on the use and execution of this protocol, please refer to Kloosterman, Erbani, et al..
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566343 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103421 | DOI Listing |
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