In the last decade, the instrumentation improvements in supercritical fluid chromatography (SFC) and the hyphenation to mass spectrometry (MS), have increased the SFC acceptance between scientists, becoming today a valuable tool in analytical chemistry. The unique selectivity, short analysis times, low consumption of organic solvents, and the greener mobile phase, have contributed to expanding its applicability which has led to an increase in the number of publications especially in the bioanalysis area. This work reviews the advantages and main applications of SFC in bioanalysis during the last 5 years. Fundamental aspects concerning mobile phase composition, stationary phase, hyphenation to MS as well as matrix effect have been discussed. Finally, the most relevant applications have been summarized.
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http://dx.doi.org/10.1002/jssc.70003 | DOI Listing |
J Chromatogr A
December 2024
I. U. CINQUIMA, Analytical Chemistry Group (TESEA), Dept. Analytical Chemistry, Faculty of Sciences, University of Valladolid 47011, Valladolid, Spain. Electronic address:
The simultaneous separation of the enantiomers of six anticoagulant rodenticides, derived from 4-hydroxycoumarin, has been studied in this work. Ten different stationary phases (zwitterionic, Pirkle-type, polysaccharides and macrocyclic antibiotics derivatives) were evaluated by using supercritical fluid chromatography coupled to two different detectors (circular dichroism and mass spectrometry-single quadrupole). The effect of the type of organic modifier and temperature on the chiral separation was investigated, and the best results were obtained with the column Regis S,S-Whelk-O1 at 25 °C when using a gradient elution program with methanol as organic modifier.
View Article and Find Full Text PDFJ Exp Med
February 2025
Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Japan.
Invariant natural killer T (iNKT) cells are unconventional T cells recognizing lipid antigens in a CD1d-restricted manner. Among these lipid antigens, α-galactosylceramide (α-GalCer), which was originally identified in marine sponges, is the most potent antigen. Although the presence of α-anomeric hexosylceramide and microbiota-derived branched α-GalCer is reported, antigenic α-GalCer has not been identified in mammals.
View Article and Find Full Text PDFAdv Mater
December 2024
Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University, Sendai, 980-8577, Japan.
Traditional selectivity of gas sensors determined by the magnitude of the response value has significant limitations. The distinctive inversion sensing behavior not only defies the traditional sensing theory but also provides insight into defining selectivity. Herein, the novel definition of selectivity is established in a study with VO(M1).
View Article and Find Full Text PDFChemosphere
December 2024
University of Washington, Mechanical Engineering Department, Seattle, WA 98195. Electronic address:
Granular activated carbon (GAC) is widely used to treat contaminated per- and polyfluoroalkyl substances (PFAS) waste streams, resulting in the accumulation of large quantities of spent GAC that need to be landfilled or regenerated. A novel modified supercritical CO (scCO) extraction for regeneration of spent GAC is developed. With the addition of organic solvents and acid modifiers, the procedure yielded > 99% perfluorooctanoic acid (PFOA) desorption after a 60-minute treatment in a continuous flow reactor.
View Article and Find Full Text PDFRSC Adv
December 2024
Department of Chemical Engineering, Marvdasht Branch, Islamic Azad University Marvdasht Iran
Supercritical fluid-based methods have been receiving increasing popularity in the production of pharmaceutical nanoparticles due to their ability to control the size and distribution of the particles and offer high purity products. The gas anti-solvent method is one of the methods in which a supercritical fluid serves as an anti-solvent. The aim of this work is to develop pazopanib hydrochloride nanoparticles as an anti-cancer agent by the supercritical GAS method.
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