Objective: This study draws on life narrative data and an intersectional framework to explore features of narratives around structural domains, aiming to better understand the possible impacts of these domains on identity.
Method: Through in-depth semi-structured interviews with 177 young adults from primarily minoritized groups (73% Asian American or Latine, 59% Women, Median Parent Income = $50,001 to $75,000), we gathered 885 life narratives. Young adults narrated a domain-general, ethnic/racial, gender, social class, and intersectional experience. Features capturing the content (Presence of Structural Domains, Connection to and Between Structural Domains) and process (Meaning Making, Affective Tone) of narratives were explored.
Results: Structural domains manifested uniquely within narratives such that ethnicity/race was discussed most frequently across narratives, whereas gender and social class were mentioned more in narratives about those domains. Additionally, Meaning Making was highest in self-defining narratives and positively correlated with the number of structural domains present within and across narratives. Affective Tone was most positive in self-defining narratives and most negative in social class narratives, which also contained the lowest Connection to Structural Domain.
Conclusion: This study combines an intersectional framework and life narrative data to understand how structural domains manifest within young adults' experiences, revealing how those domains are interconnected and may impact identity.
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http://dx.doi.org/10.1111/jopy.12984 | DOI Listing |
Nat Commun
December 2024
Architecture and Dynamics of Biological Macromolecules, Institut Pasteur, Université Paris Cité, CNRS UMR 3528, Paris, France.
Replication Protein A (RPA) plays a pivotal role in DNA replication by coating and protecting exposed single-stranded DNA, and acting as a molecular hub that recruits additional replication factors. We demonstrate that archaeal RPA hosts a winged-helix domain (WH) that interacts with two key actors of the replisome: the DNA primase (PriSL) and the replicative DNA polymerase (PolD). Using an integrative structural biology approach, combining nuclear magnetic resonance, X-ray crystallography and cryo-electron microscopy, we unveil how RPA interacts with PriSL and PolD through two distinct surfaces of the WH domain: an evolutionarily conserved interface and a novel binding site.
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December 2024
Department of Biomedicine, Aarhus University, 8000, Aarhus C, Denmark.
CD163, a macrophage-specific receptor, plays a critical role in scavenging hemoglobin released during hemolysis, protecting against oxidative effects of heme iron. In the bloodstream, hemoglobin is bound by haptoglobin, leading to its immediate endocytosis by CD163. While haptoglobin's structure and function are well understood, CD163's structure and its interaction with the haptoglobin-hemoglobin complex have remained elusive.
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December 2024
Laboratory of Retrovirology, The Rockefeller University, New York, NY, 10065, USA.
ZAP is an antiviral protein that binds to and depletes viral RNA, which is often distinguished from vertebrate host RNA by its elevated CpG content. Two ZAP cofactors, TRIM25 and KHNYN, have activities that are poorly understood. Here, we show that functional interactions between ZAP, TRIM25 and KHNYN involve multiple domains of each protein, and that the ability of TRIM25 to multimerize via its RING domain augments ZAP activity and specificity.
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December 2024
Beijing Frontier Research Center for Biological Structure, State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Exceptionally diverse type V CRISPR-Cas systems provide numerous RNA-guided nucleases as powerful tools for DNA manipulation. Two known Cas12e nucleases, DpbCas12e and PlmCas12e, are both effective in genome editing. However, many differences exist in their in vitro dsDNA cleavage activities, reflecting the diversity in Cas12e's enzymatic properties.
View Article and Find Full Text PDFAnn Clin Transl Neurol
December 2024
Department of Pediatrics, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, P. R. China.
Background: Variants in the GABRA2 gene, which encodes the α2 subunit of the γ-aminobutyric acid A receptor, have been linked to a rare form of developmental and epileptic encephalopathy (DEE) referred to as DEE78. Only eight patients have been reported globally. This study presents the clinical presentation and genetic analysis of a Chinese family with a child diagnosed with DEE78, due to a novel GABRA2 variant.
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