Folic acid functionalized Ag@MOF(Ag) decorated carboxymethyl starch nanoparticles as a new doxorubicin delivery system with inherent antibacterial activity.

Considering the benefits of controlled drug delivery in cancer treatment, as well as the importance of biological macromolecules in this area, herein, the pre-synthesized carboxymethyl starch (CMS) was converted to CMS nanoparticles (CMS NPs) in one easy nanoprecipitation way. Thereafter, the Ag@MOF(Ag) was in situ synthesized in the presence of pre-prepared CMS NPs (CMS NPs/Ag@MOF(Ag)). Eventually, the functionalization with folic acid (FA) obtained the CMS NPs/Ag@MOF(Ag)-FA. The success of the accomplished process was approved by doing several techniques, including FT-IR, XRD, EDX, AFM, etc. The SEM analysis showed a combination of rod-like and spherical-like morphology for the fabricated bio-nanocomposite. The generated CMS NPs/Ag@MOF(Ag)-FA with a surface area of 10.595 m/g displayed a pore size of 13.666 nm and 82.99 % of doxorubicin (DOX) loading efficiency (DOX@CMS NPs/Ag@MOF(Ag)-FA). The 38.46 % and 58.19 % of loaded DOX were released respectively within 240 h at pH 7.4 and pH 5.0, referring to the pH-responsivity of the constructed system. 27.25 % of inhibitory effects on HeLa cells were obtained for the drug-loaded bio-nanocomposite. The CMS NPs/Ag@MOF(Ag)-FA also displayed an inherent antibacterial activity towards two common gram-negative and gram-positive bacteria. All of these results can contribute to developing polysaccharide-based porous systems in controlled cancer therapy.