Multifocal structured illumination microscopy (MSIM) is a popular super-resolution imaging technique known for its good probe compatibility, low laser power requirements, and improved imaging depth, making it widely applicable in biomedical research. However, the speed of MSIM imaging is typically constrained by the approaches employed to generate and scan the laser foci across the sample. In this study, we propose a flexible two-photon excitation MSIM method using a pair of acousto-optic deflectors. By adopting addressable scanning (AS) and synchronized capturing, MSIM super-resolution imaging can be performed in multiple discrete regions of interest (ROIs) within the field of view. Notably, this AS-MSIM scheme not only enhances the speed of MSIM imaging but also alleviates photobleaching and phototoxicity to biological samples. We demonstrate its potential by achieving super-resolution imaging of selected mitochondria within cells at a frame rate of 4 Hz. Furthermore, we deliberate the possibility of even faster imaging.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/OL.538097 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Super-Resolved Mapping of Electrochemical Reactivity in Single 3D Catalysts.
Nano Lett
January 2025
Institute of Nanochemistry and Nanobiology, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, P.R. China.
Crystals with three-dimensional (3D) stereoscopic structures, characterized by diverse shapes, crystallographic planes, and morphologies, represent a significant advancement in catalysis. Differentiating and quantifying the catalytic activity of specific surface facets and sites at the single-particle level is essential for understanding and predicting catalytic performance. This study employs super-resolution radial fluctuations electrogenerated chemiluminescence microscopy (SRRF-ECLM) to achieve high-resolution mapping of electrocatalytic activity on individual 3D CuO crystals, including cubic, octahedral, and truncated octahedral structures.
View Article and Find Full Text PDFBacterial strains that are genetically engineered to constitutively produce fluorescent proteins have aided our study of bacterial physiology, biofilm formation, and interspecies interactions. Here, we report on the construction and utilization of new strains that produce the blue fluorescent protein mTagBFP2, the green fluorescent protein sfGFP, and the red fluorescent protein mScarlet-I3 in species , and . Gene fragments, developed to contain the constitutive promoter P , the fluorescent gene of interest as well as , providing resistance to the antibiotic spectinomycin, were inserted into selected open reading frames on the chromosome that were both transcriptionally silent and whose loss caused no measurable changes in fitness.
View Article and Find Full Text PDFSuper-Resolution Mitochondrial Fluorescent Probe for Accurate Monitoring of Drug-Induced Liver Injury.
Anal Chem
January 2025
Shandong Provincial Key Laboratory of Tumor Imaging Equipment Development and Integrated Diagnosis and Treatment Technology, Linyi University, Linyi 276000, China.
Drug-induced liver injury (DILI) has emerged as an urgent clinical challenge. It is characterized by mitochondrial dysfunction in liver cells, which leads to abnormal changes in HO levels within the mitochondria. Super-resolution imaging allows for the observation of the fine structure of mitochondria at the nanometer scale, potentially enabling the detection of mitochondrial HO levels during DILI at the subcellular organelle level.
View Article and Find Full Text PDFSuper-resolution imaging of proteins inside live mammalian cells with mLIVE-PAINT.
Protein Sci
February 2025
IRR Chemistry Hub, Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh, UK.
Super-resolution microscopy has revolutionized biological imaging, enabling the visualization of structures at the nanometer length scale. Its application in live cells, however, has remained challenging. To address this, we adapted LIVE-PAINT, an approach we established in yeast, for application in live mammalian cells.
View Article and Find Full Text PDFMetasurface-Coated Liquid Microlens for Super Resolution Imaging.
Micromachines (Basel)
December 2024
State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an 710054, China.
Inspired by metasurfaces' control over light fields, this study created a liquid microlens coated with a layer of Au@TiO, Core-Shell nanospheres. Utilizing the surface plasmon resonance (SPR) effect of Au@TiO, Core-Shell nanospheres, and the formation of photonic nanojets (PNJs), this study aimed to extend the imaging system's cutoff frequency, improve microlens focusing, enhance the capture capability of evanescent waves, and utilize nanospheres to improve the conversion of evanescent waves into propagating waves, thus boosting the liquid microlens's super-resolution capabilities. The finite difference time domain (FDTD) method analyzed the impact of parameters including nanosphere size, microlens sample contact width, and droplet's initial contact angle on super-resolution imaging.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!