AI Article Synopsis
- Genotyping is the analysis of an organism's DNA to determine its genetic traits and has applications in fields like agriculture, biomedicine, and conservation.
- Genetic markers are categorized into random markers (like RFLP and AFLP) and functional markers (like SCoT and SRAP), with the latter being less utilized in animal science despite their advantages.
- This review provides an overview of the different genotyping markers available, their benefits and limitations, and emphasizes the need for more use of functional markers in genetic research.
Article Abstract
Genotyping is the process of determining the genetic makeup of an organism by examining its DNA sequences using various genetic markers. It has been widely used in various fields, such as agriculture, biomedical and conservation research, to study genetic diversity, inheritance, the genetic basis of disease-associated traits, evolution, adaptation, etc., Genotyping markers have evolved immensely and are broadly classified as random markers (RFLP, RAPD, AFLP, etc.) and functional markers (SCoT, CDDP, SRAP, etc.). However, functional markers are very limited in genotype studies, especially in animal science, despite their advantages in overcoming the limitations of random markers, which are directly linked with phenotypic traits, high specificity, and similar logistic requirements. The current review surveyed the available random and functional markers for genotyping applications, focusing on livestock including plant and microbe domains. This review article summarises the application, advantages, and limitations of developed markers and methods for genotyping applications. This review aims to make the reader aware of all available markers, their design principles, and methods, and we discuss the marker inheritance patterns of RLFP and AFLP. The review further outlines the marker selection for particular applications and endorses the application of functional markers in genotyping research.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11524813 | PMC |
| http://dx.doi.org/10.3389/fgene.2024.1463474 | DOI Listing |
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