A total of 52 Pasteurella multocida strains of capsular serogroups (A, B and D) were screened for anti-quorum sensing activity against Chromobacterium violaceum. Of which, 12 strains of serogroups A were found to possess anti-quorum sensing activity. Inhibition activity was highest for strain NIVEDIPm9 and lowest for strain NIVEDIPm30 based on zone of pigment inhibition. Further, cell free extract of NIVEDIPm9 strain showed highest anti-biofilm activity in reference E. coli strain and concentration dependent degradation activity of C6-AHL molecule. In whole genome sequence annotation of NIVEDIPm9 strain predicted the presence of four metallo-β-lactamases (MBL) fold metallo-hydrolase proteins. In docking studies, MBL1 and MBL3 proteins showed high binding affinity with autoinduce signalling molecules AHL compound of OH-C10, binding energy value were -6.3 and -6.2 kcal/mol. Interaction study of VAF and quorum sensing molecules showed that OmpA and HgbA proteins were stimulated by all the ten molecules (C4-AHLs, C6-AHLs, C10-AHLs, C14-AHLs, 3-oxo-C10-AHLs, 3OH-C10-HSL, C8-HSL, C10-HSL, C12-HSL, C14-HSL), while toxA gene was stimulated by OH-C10-AHL molecule, sodC gene was stimulated by none. In conclusion, we described the anti-quorum sensing activities of diverse P. multocida strains causing Pasteurellosis in livestock.
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http://dx.doi.org/10.1016/j.micpath.2024.107085 | DOI Listing |
Mol Biol Rep
November 2024
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Microb Pathog
December 2024
ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Post Box No. 6450, Yelahanka, Bengaluru, 560064, Karnataka, India. Electronic address:
A total of 52 Pasteurella multocida strains of capsular serogroups (A, B and D) were screened for anti-quorum sensing activity against Chromobacterium violaceum. Of which, 12 strains of serogroups A were found to possess anti-quorum sensing activity. Inhibition activity was highest for strain NIVEDIPm9 and lowest for strain NIVEDIPm30 based on zone of pigment inhibition.
View Article and Find Full Text PDFBraz J Microbiol
October 2024
Nitte (Deemed to be University), Nitte University Centre for Science Education and Research, Paneer Campus, Deralakatte Mangaluru, 575018, Karnataka, India.
The increasing resistance of microbes to conventional drugs is a serious problem worldwide that has increased the need for alternative antimicrobial compounds. Naturally occurring essential oils (EOs) are considered an important component of traditional pharmacopeia because of their antimicrobial and antioxidant properties. This has attracted researchers to identify novel therapeutic anti-pathogenic agents that could act as non-toxic quorum sensing inhibitors, thus controlling infections without encouraging the development of bacterial resistance.
View Article and Find Full Text PDFVirulence
December 2024
Department of Laboratory Medicine, The Third Xiangya Hospital of Central South University, Changsha, China.
BMC Microbiol
October 2024
Department of Biology Education, Faculty of Teacher Training and Education, Khairun University, Ternate, 97728, Indonesia.
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