Purpose: We assessed feasibility and satisfaction of the Active Children Through In-Home Web-Based Physical Activity pilot trial, delivering a web-based movement integration intervention to children.
Method: Eighty-two children (8-11 y), insufficiently active, were randomly assigned to either exercise intervention (n = 41) or wait-list control (n = 41). The intervention involved 20-minute exercise videos, 5 times weekly for 12 weeks, using the UNICEF Kid Power website at home. Feasibility metrics included recruitment (target: 70%), retention (target: 80%), adherence rates, and satisfaction assessed through surveys and interviews. Retention rate-1 was percentage completing posttest surveys, and retention rate-2 was percentage completing posttest accelerometry. Parent logs assessed adherence.
Results: Recruitment, retention-1, and retention-2 rates were 73.6%, 93.9%, and 80.5%, respectively. The intervention group had 5 dropouts; wait-list control had none. Sixty-nine percent showed high intervention adherence. Parents consistently expressed satisfaction, finding the intervention enjoyable and beneficial. Although children initially provided positive reviews, their interest declined over time, with increasing expressions of monotony. Suggestions to increase novelty and incorporate a social component were made by participants. The intervention was also found to be particularly useful during inclement weather.
Conclusion: Active Children Through In-Home Web-Based Physical Activity trial exceeded feasibility targets of recruitment and retention and achieved moderate overall adherence. Future trials should emphasize novelty and peer participation for improved adherence and satisfaction.
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http://dx.doi.org/10.1123/pes.2024-0030 | DOI Listing |
Sci Rep
December 2024
School of Physical Education, Shanghai University of Sport, Shanghai, 200438, China.
Objective: This study aimed to examine the levels of physical activity (PA), sleep, and mental health (MH), specifically depression, anxiety, and stress, among Chinese university students. It also aimed to analyze the influencing factors of MH, providing a theoretical foundation for developing intervention programs to improve college students' mental health.
Methods: A stratified, clustered, and phased sampling method was employed.
Sci Rep
December 2024
Department of Psychiatry and Behavioral Sciences and Weill Center for Neurosciences, University of California, San Francisco, CA, 94107, USA.
Telomere attrition is a hallmark of biological aging, contributing to cellular replicative senescence. However, few studies have examined the determinants of telomere attrition in vivo in humans. Mitochondrial Health Index (MHI), a composite marker integrating mitochondrial energy-transformation capacity and content, may be one important mediator of telomere attrition, as it could impact telomerase activity, a direct regulator of telomere maintenance.
View Article and Find Full Text PDFNat Commun
December 2024
Department of Genetics, Yale University, Yale School of Medicine, New Haven, 06510, CT, USA.
The cis-regulatory elements encoded in an mRNA determine its stability and translational output. While there has been a considerable effort to understand the factors driving mRNA stability, the regulatory frameworks governing translational control remain more elusive. We have developed a novel massively parallel reporter assay (MPRA) to measure mRNA translation, named Nascent Peptide Translating Ribosome Affinity Purification (NaP-TRAP).
View Article and Find Full Text PDFNat Commun
December 2024
Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
The mechanism(s) underlying gut microbial metabolite (GMM) contribution towards alcohol-mediated cardiovascular disease (CVD) is unknown. Herein we observe elevation in circulating phenylacetylglutamine (PAGln), a known CVD-associated GMM, in individuals living with alcohol use disorder. In a male murine binge-on-chronic alcohol model, we confirm gut microbial reorganization, elevation in PAGln levels, and the presence of cardiovascular pathophysiology.
View Article and Find Full Text PDFNat Commun
December 2024
Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, USA.
Programmable and modular systems capable of orthogonal genomic and transcriptomic perturbations are crucial for biological research and treating human genetic diseases. Here, we present the minimal versatile genetic perturbation technology (mvGPT), a flexible toolkit designed for simultaneous and orthogonal gene editing, activation, and repression in human cells. The mvGPT combines an engineered compact prime editor (PE), a fusion activator MS2-p65-HSF1 (MPH), and a drive-and-process multiplex array that produces RNAs tailored to different types of genetic perturbation.
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