To enhance the understanding of flocculated sludge granulation, this study focused on a bacterial denitrification system using low-intensity ultrasound and inert particles to stimulate cell activity and facilitate flocculated sludge granulation. After 75 days, the activated carbon, activated carbon + ultrasonication, and microplastic + ultrasonication groups showed partial pelletization. Both ultrasound and inert particles promoted extracellular polymeric substance secretion and enhanced electron transport system activity. Low-intensity ultrasound improved denitrification performance and enhanced denitrifying bacteria. The addition of inert materials facilitated denitrifying flocculated sludge granulation. Low-intensity ultrasound combined with microplastics obtained the highest activity and enrichment of denitrifying bacteria in granular sludge. This study provides new ideas for optimizing anaerobic sludge granulation.

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http://dx.doi.org/10.1016/j.biortech.2024.131724DOI Listing

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