AI Article Synopsis
- A new mass spectrometry imaging technique allows for the detection and analysis of glycosaminoglycans (GAGs) in retinal tissue, overcoming previous limitations in spatial resolution.
- By using chondroitinase enzymes, researchers can extract and analyze various GAG oligosaccharides, ranging from disaccharides to hexasaccharides, directly from tissue sections.
- The study showcases the ability to resolve isomeric GAG oligosaccharides and their sulfation states across different tissue regions using a method called trapped ion mobility spectrometry (TIMS).
Article Abstract
Previously, spatially resolved analysis of glycosaminoglycans (GAGs), by type and sulfation state, was unobtainable. Here, we describe a mass spectrometry imaging (MSI) approach which enables the detection, identification, localization, and profiling of GAG oligosaccharides directly from retinal tissue. Through treatment of tissues with relevant chondroitinase enzymes, we liberate and spatially resolve chondroitin, dermatan, and hyaluronan from disaccharides through to hexasaccharides, directly from tissue sections. We demonstrate the separation of isomeric GAG oligosaccharide ions at different histologically relevant regions using trapped ion mobility spectrometry (TIMS). This paper describes the first spatially resolved analysis of multiple GAGs and their oligosaccharide sulfation state(s) directly from tissues.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11561879 | PMC |
| http://dx.doi.org/10.1021/acs.analchem.4c02706 | DOI Listing |
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