Stimulus-responsive magnetic enzyme-encapsulated coordination polymer for the potential field monitoring of alkaline phosphatase using personal glucometer.

Alkaline phosphatase (ALP) is an enzyme that can hydrolyze phosphate groups, and its activity is related to algal bloom outbreaks in eutrophic waters. A magnetic ALP-responsive material named FeO@INV/Zn-AMP was synthesized at room temperature by using zinc ions and adenosine monophosphate (AMP) as building blocks integrated invertase (INV). When ALP is present, it hydrolyzes the phosphate group of AMP, causing the disassembly of the FeO@INV/Zn-AMP material and the release of signaling molecule (INV). After magnet separation, the released INV can digest sucrose into glucose measured by a personal glucometer (PGM), providing a signal that indicates the activity of ALP. This method allows for selective, sensitive, and easy detection of ALP in field settings without the need for complicated labeling operations. Benefit from the selective stimulus-response and the enzyme catalyzed amplification, a simple and portable method for the monitoring of ALP using PGM was established, with linear range of 0.005-0.2 U/mL and a detection limit of 0.001 U/mL, which showed the potential of field monitoring for alkaline phosphatase in the future.