Elucidating enantioselective fate and sensitive biomarkers in zebrafish of chiral pesticide fenpropidin: Insights into metabolic pathways and hazard assessment.

Fenpropidin (FPD), a widely utilized chiral fungicide, has been detected in aquatic environments. This study systematically evaluated the bioaccumulation, depuration, biotransformation, and sensitive biomarkers of FPD enantiomers in zebrafish to assess their environmental risks. Compared with S-FPD, R-FPD demonstrated a higher rate of enrichment and an increased level of bioaccumulation. The half-lives of R-FPD and S-FPD were 0.49 ± 0.01 and 0.91 ± 0.02 days at 0.05 mg/L and 1.65 ± 0.01 and 1.85 ± 0.03 days at 0.5 mg/L. Nontarget metabolism analysis identified nine metabolites, primarily formed through hydroxylation, oxidation, dehydration, glutathione conjugation, and glucuronidation pathways. Some metabolites exhibited high toxicity, underscoring the necessity for continuous monitoring of their toxicological effects and environmental fate in risk assessments. The toxicity of S-FPD in zebrafish was 1.21 times greater than that of R-FPD. Furthermore, this study identified sensitive markers for the enantiomers at both protein and transcriptional levels using an integrated biomarker response approach. S-FPD exhibited increased sensitivity to apoptosis and metabolic gene expression, while R-FPD showed greater sensitivity to antioxidant kinase activity. These findings facilitate timely monitoring of environmental pollution caused by FPD enantiomers. This study provides critical insights for assessing potential risks associated with pesticide exposure to human health.