AI Article Synopsis
- * Research utilizing RNA-seq data and in situ hybridization showed that Odz4 is specifically expressed in the sinoatrial node (SAN) and the cardiac conduction system (CCS).
- * Experiments using an inducible system to express Odz4's intracellular domain demonstrated that it enhances the development of pacemaker-like cells, suggesting its importance in SAN differentiation and biological pacemaker development.
Article Abstract
Cardiac arrhythmias stemming from abnormal sinoatrial node (SAN) function can lead to sudden death. Developing a biological pacemaker device for treating sick sinus syndrome (SSS) could offer a potential cure. Understanding SAN differentiation is crucial, yet its regulatory mechanism remains unclear. We reanalyzed published RNA-seq data and identified Odz4 as a SAN-specific candidate. In situ hybridization revealed Odz4 expression in the cardiac crescent and throughout the cardiac conduction system (CCS). To assess the role of Odz4 in CCS differentiation, we utilized a Tet-Off inducible system for its intracellular domain (ICD). Embryonic bodies (EBs) exogenously expressing Odz4-ICD exhibited an increased propensity to develop into pacemaker-like cells with enhanced automaticity and upregulated expression of SAN-specific genes. CellChat and GO analyses unveiled SAN-specific enrichment of ligand-receptor sets, especially Ptn-Ncl, and extracellular matrix components in the group exogenously expressing Odz4-ICD. Our findings underscore the significance of Odz4 in SAN development and offer fresh insights into biological pacemaker establishment.
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Odz4 upregulates SAN-specific genes to promote differentiation into cardiac pacemaker-like cells.
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- * Experiments using an inducible system to express Odz4's intracellular domain demonstrated that it enhances the development of pacemaker-like cells, suggesting its importance in SAN differentiation and biological pacemaker development.
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