eDNA technology reveals fish species diversity and ecological corridor function in large raft mussel aquaculture area in the East China Sea.

Human activities have transformed coastal environments by introducing numerous artificial structures that impact local ecosystems. Our study investigated fish species diversity in the mussel farm (MF) and the surrounding natural reef habitat (NH) around Gouqi Island in the East China Sea (ECS). Gouqi Island, which hosts the largest mussel farm in ESC, is also a critical region for marine fish activity. We monitored fish species diversity across four seasons for the first time using eDNA technology in December (winter) 2022, March (spring), June (summer), and September (autumn) 2023. We recorded a total of 55 fish species across 49 genera, 33 families, and 10 orders. Alpha diversity analysis revealed that species diversity peaked in summer throughout the year. Diversity in the mussel farming area was significantly higher than in the natural reef habitat. Principal Component Analysis (PCA) and Analysis of Similarities (ANOSIM) confirmed significant differences in community composition between the two habitats (p < 0.01), with a more stable community structure in the mussel farm. This indicates that the mussel farming area supports a distinct fish community compared to the surrounding natural waters. The dominant fish family in the mussel farm was Sciaenidae, which includes several economically valuable species and could be a target for resource conservation. Additionally, the mussel farm hosted numerous migratory fish species exhibiting clear seasonal patterns, suggesting that the farm serves as an important ecological corridor utilized by these species throughout their life cycles.