AI Article Synopsis
- The protocol outlines a method for assessing the fitness of the oral symbiont A. aphrophilus related to its type VI secretion system (T6SS) using competition assays and metaproteomics.
- It includes guidelines for designing T6SS-specific mutants and conducting competition tests with the pathobiont A. actinomycetemcomitans in biofilm models.
- The biofilm model simulates the oral plaque ecosystem, featuring seven species, and for detailed procedures, it refers to the work of Oscarsson et al.
Article Abstract
Here, we present a protocol for evaluating type VI secretion system (T6SS)-dependent fitness of the oral symbiont A. aphrophilus using biofilm competition assays and metaproteomics. We describe steps for designing T6SS-specific mutants. We then detail procedures for using them in competition assays with the pathobiont A. actinomycetemcomitans and in biofilm models, analyzing metaproteomes to assess the impact of the T6SS on multiple pathobionts. The biofilm model is designed to mimic the oral plaque ecosystem and includes seven species. For complete details on the use and execution of this protocol, please refer to Oscarsson et al..
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543863 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103415 | DOI Listing |
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