AI Article Synopsis
- The study focuses on canine distemper virus (CDV), which is highly contagious among domestic and wild animals, highlighting the necessity for updated vaccination strategies due to emerging strains.
- Researchers tested a new generation of vaccines using CDV peptides on mice, observing significant immune responses and safety compared to a placebo.
- The findings suggest that these peptide-based vaccines could improve current vaccination methods and help control CDV in both domestic dogs and wildlife.
Article Abstract
Background: is the etiological agent of a highly contagious disease that affects diverse domestic and wild animals. Vaccination is considered the most suitable strategy for controlling CDV dissemination, transmission, and distemper disease. However, the emergence of new CDV strains has led to the need to update the current vaccine strategies employed to prevent CDV infection in domestic and wild animals. Currently, there is a lack of effective alternatives for wild animals. Diverse computational tools, especially peptide-based therapies, enable the development of new universal vaccines.
Objective: The aim of this study was to evaluate the safety and humoral and cellular immune response of a new generation of vaccines based on CDV peptides as single-peptide mixtures or multiepitope CDV polypeptides in mice.
Methods: Twenty-four BALB/c mice were subjected to a three-dose regimen for 28 days. Seroconversion was evaluated via ELISA, and cellular immune responses were evaluated via flow cytometry through activation-induced markers (AIMs).
Results: Compared with the placebo, the peptide mixture and multiepitope CDV polypeptide were safe, and seroconversion was statistically significant in the multiepitope CDV polypeptide and commercial vaccine (CV) groups. The numbers of antigen-specific CD4+CD134+ and IFN-γ+ T cells, CD8+ T cells and TNF-α- and IL-6-producing cells were greater in the mice immunized with the multiepitope CDV polypeptide than in the control mice.
Conclusion: This combined approach represents a potential step forward in developing new immunization candidates or enhancing current commercial vaccines to control CDV disease in domestic dogs and wild animals.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511104 | PMC |
| http://dx.doi.org/10.3390/vaccines12101140 | DOI Listing |
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