This study focused on optimizing a cold water extraction method to obtain bioactive compounds from chamomile ( L.), addressing increasing consumer demand for natural products and nutraceuticals. A full-factorial design was employed to evaluate the effects of temperature, time, and chamomile amount on the polyphenolic profile of extracts. The samples were characterized by HPLC-DAD and UV-Vis coupled with chemometrics; the analysis showed that extraction time negatively affected extract quality, as did the interaction between time and temperature. In addition, a significant positive quadratic effect for temperature and a positive coefficient for chamomile amount was found. ASCA was used to assess the UV-Vis profile, offering an alternative untargeted method for understanding the variable effects. The optimal extraction conditions (25 °C, 32 min, and 2.5 g of chamomile) produced samples high in hydroxybenzoic and hydroxycinnamic acids and flavanol derivatives. Using A face-centered design, this study also monitored antioxidant activity via a DPPH scavenging assay, confirming that the optimal conditions yielded samples within the range of maximum antioxidant activity in the studied experimental domain.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510547 | PMC |
| http://dx.doi.org/10.3390/molecules29204925 | DOI Listing |
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