AI Article Synopsis
- L. Webb ex Prantl is widely used in traditional medicine but lacks an effective genetic transformation system for research and application.
- This study presents a new, efficient method for genetic transformation that involves dipping inflorescences in a specific solution for 45 seconds, leading to successful plant transformations.
- The method also incorporates CRISPR/Cas9 for targeted genome editing, enabling the creation of mutant plants with a specific trait in just 2.5 months.
Article Abstract
L. Webb ex Prantl is used in traditional medicine globally. However, the lack of an efficient and reliable genetic transformation system has seriously limited the investigation of gene function and further utilization of . In this study, a highly efficient, time-saving, and cost-effective -mediated genetic transformation system has been developed in . In this method, the transformation was accomplished by simply dipping developing inflorescences for 45 s into an suspension (OD = 0.6) containing 5% sucrose and 0.03% (/) Silwet L-77. Treated plants were allowed to set seeds which were then plated on a selective medium with hygromycin B (HygB) to screen transformants. Additionally, the CRISPR/Cas9 genomic editing system was validated by targeting phytoene desaturase () gene using this floral dip method, and mutant plants with the expected albino phenotype could be obtained in 2.5 months. This genetic transformation and targeted editing system will be a valuable tool for routine investigation of gene function and further exploitation in .
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510603 | PMC |
| http://dx.doi.org/10.3390/plants13202833 | DOI Listing |
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