AI Article Synopsis

  • Group B Streptococcus (GBS) is a common bacterium that can become harmful to newborns, pregnant women, and the elderly, and its biofilm formation is crucial for its pathogenicity.
  • Researchers created a model to study GBS biofilm formation using human fibrinogen (hFg), finding that this leads to a robust biofilm structure supported by proteins and extracellular DNA (eDNA).
  • The study revealed that the SaeRS regulatory system is essential for biofilm development, with its absence significantly hindering biomass and disrupting biofilm structure.

Article Abstract

, also known as Group B or GBS, is a commensal colonizer of human vaginal and gastrointestinal tracts that can also be a deadly pathogen for newborns, pregnant women, and the elderly. The SaeRS two-component regulatory system (TCS) positively regulates the expression of two GBS adhesins genes, but its role in the formation of biofilm, an important step in pathogenesis, has not been investigated. In the present study, we set up a novel model of GBS biofilm formation using surfaces coated with human fibrinogen (hFg). Biofilm mass and structure were analyzed by crystal violet staining and three-dimensional fluorescence microscopy, respectively. GBS growth on hFg resulted in the formation of a mature and abundant biofilm composed of bacterial cells and an extracellular matrix containing polysaccharides, proteins, and extracellular DNA (eDNA). Enzymatic and genetic analysis showed that GBS biofilm formation on hFg is dependent on proteins and eDNA in the extracellular matrix and on the presence of covalently linked cell wall proteins on the bacterial surface but not on the type-specific capsular polysaccharide. In the absence of the SaeR regulator of the SaeRS TCS, there was a significant reduction in biomass formation, with reduced numbers of bacterial cells, reduced eDNA content, and disruption of the biofilm architecture. Overall, our data suggest that GBS binding to hFg contributes to biofilm formation and that the SaeRS TCS plays an important role in this process.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510217PMC
http://dx.doi.org/10.3390/microorganisms12102096DOI Listing

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