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Analysis of Azathioprine Metabolites in Autoimmune Hepatitis Patient Blood-Method Development and Validation. | LitMetric

Analysis of Azathioprine Metabolites in Autoimmune Hepatitis Patient Blood-Method Development and Validation.

Int J Mol Sci

Proteomics Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary.

Published: October 2024

AI Article Synopsis

  • Autoimmune hepatitis (AIH) is a chronic liver disease treated with steroids and the thiopurine drug azathioprine (AZA), which is metabolized into active forms like 6-thioguanine (6-TG) and 6-methyl-mercaptopurine (6-MMP).
  • This study focused on creating a quick and effective ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method to analyze levels of 6-TG and 6-MMP in blood samples from AIH patients.
  • The new method was validated following EMA guidelines and proved to be accurate and precise for monitoring these important metabolites, facilitating better treatment management for patients.

Article Abstract

Autoimmune hepatitis (AIH) is a chronic inflammatory liver disease treated by steroids and immunomodulator thiopurine drugs such as azathioprine (AZA). AZA is metabolized in the human body into bioactive forms such as 6-thioguanine (6-TG) and 6-methyl-mercaptopurine (6-MMP). Monitoring the levels of bioactive AZA metabolites is very important for proper treatment of patients. In this study, our aim was to develop and validate a fast and sensitive ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method for the analysis of 6-TG and 6-MMP from blood samples of patients with AIH to monitor the level of these bioactive metabolites. The detection and quantification of the analytes was carried out by Selected Reaction Monitoring (SRM)-based targeted mass spectrometry. The method was validated according to the EMA guidelines. Blood samples from patients with AIH treated with AZA were analysed with the developed method. The method was successfully validated with appropriate accuracy and precision for the target biomolecules and their concentration in the samples from patients with AIH was determined. The developed and validated UHPLC-MS method enables the fast and precise analysis of AZA metabolites.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508274PMC
http://dx.doi.org/10.3390/ijms252011233DOI Listing

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