Transcriptomic Analysis of the Response of the Larva Midgut to 2913 Infection.

Int J Mol Sci

Key Laboratory for Sustainable Forest Ecosystem Management of Ministry of Education, College of Forestry, Northeast Forestry University, Harbin 150040, China.

Published: October 2024

AI Article Synopsis

  • - The study focuses on the Lepidoptera pest Denis Schiffermuller, which harms Pinaceae plants, and analyzes the midgut transcriptome of larvae infected with strain 2913 over various time intervals, identifying 7497 differentially expressed genes (DEGs) related to the infection.
  • - Among these DEGs, several genes linked to toxin activation, receptor proteins for insecticidal proteins, and metabolic detoxification were highlighted, suggesting a complex response mechanism in the larvae to counteract infection over time.
  • - The research indicates that enzymes like trypsin and chymotrypsin play roles in activating and degrading toxins, while certain protein expressions may either enhance or weaken the larvae's resistance to the pest strain

Article Abstract

Denis Schiffermuller (Lepidoptera: Pyralidae) is an oligophagous pest that mainly damages Pinaceae plants. Here, we investigated the effects of the 2913 strain ( 2913), which carries the 1Ac, 2Ab, and 3Aa genes, on the midgut transcriptome at 6, 12, and 24 h after infection. In total, 7497 differentially expressed genes (DEGs) were identified from the midgut transcriptome of larvae infected with 2913. Among these DEGs, we identified genes possibly involved in 2913-induced perforation of the larval midgut. For example, the DEGs included 67 genes encoding midgut proteases involved in / toxin activation, 74 genes encoding potential receptor proteins that bind to insecticidal proteins, and 19 genes encoding receptor NADH dehydrogenases that may bind to 1Ac. Among the three transcriptomes, 88 genes related to metabolic detoxification and 98 genes related to immune defense against 2913 infection were identified. Interestingly, 145 genes related to the 60S ribosomal protein were among the DEGs identified in the three transcriptomes. Furthermore, we performed bioinformatic analysis of zonadhesin, GST, CYP450, and CarE in the midgut to determine their possible associations with 2913. On the basis of the results of this analysis, we speculated that trypsin and other serine proteases in the larval midgut began to activate / prototoxin at 6 h to 12 h after 2913 ingestion. At 12 h after 2913 ingestion, chymotrypsin was potentially involved in degrading the active core fragment of 3Aa toxin, and the detoxification enzymes in the larvae contributed to the metabolic detoxification of the toxin. The ABC transporter and several other receptor-protein-related genes were also downregulated to increase resistance to 2913. However, the upregulation of 60S ribosomal protein and heat shock protein expression weakened the resistance of larvae to 2913, thereby enhancing the expression of NADH dehydrogenase and other receptor proteins that are highly expressed in the larval midgut and bind to activating toxins, including 1Ac. At 24 h after 2913 ingestion, many activated toxins were bound to receptor proteins such as APN in the larval midgut, resulting in membrane perforation. Here, we clarified the mechanism of 2913 infection in larvae, as well as the larval immune defense response to 2913, which provides a theoretical basis for the subsequent control of using .

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11507524PMC
http://dx.doi.org/10.3390/ijms252010921DOI Listing

Publication Analysis

Top Keywords

larval midgut
16
0
13
2913 infection
12
degs identified
12
genes encoding
12
receptor proteins
12
2913 ingestion
12
genes
10
midgut
9
midgut transcriptome
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!