AI Article Synopsis
- A detailed metabolite profiling of a medicinal plant using UHPLC-ESI-MS/MS was performed for the first time, identifying 71 compounds, mostly flavonoids, triterpene glycosides, and ecdysteroids.
- The compounds schaftoside, 26-hydroxyecdysone, and silviridoside were highlighted as important markers for evaluating preparation quality.
- The methanol extract demonstrated cytotoxic and Wnt pathway-inhibiting effects against triple-negative breast cancer, identifying 2-Deoxy-20-hydroxyecdysone as potent, while the presence of a hydroxyl group at C-2 in ecdysteroids was linked to reduced cytotoxicity against cancer cells.
Article Abstract
A comprehensive metabolite profiling of the medicinal plant using an UHPLC-ESI-MS/MS method is described for the first time. A total of 71 compounds were identified and annotated, the most common of which were flavonoids, triterpene glycosides, and ecdysteroids. The three major compounds schaftoside, 26-hydroxyecdysone, and silviridoside can be chosen as the markers for the assessment of the quality of preparations. The methanol extract and a variety of metabolites identified in were screened for their cytotoxic and Wnt pathway-inhibiting activities against triple-negative breast cancer (TNBC), the deadliest form of cancer in women. 2-Deoxy-20-hydroxyecdysone with submicromolar IC was identified as a result. The structure-activity relationship derived from the data from the in vitro proliferation assay showed that the hydroxyl group present at position C-2 of steroid core reduces the ecdysteroids' cytotoxicity against cancer cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505650 | PMC |
| http://dx.doi.org/10.3390/biom14101285 | DOI Listing |
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