Designing a multi-epitope influenza vaccine: an immunoinformatics approach.

Sci Rep

Department of Biology, Faculty of Science, Shahrekord University, Shahrekord, Iran.

Published: October 2024

Influenza continues to be one of the top public health problems since it creates annual epidemics and can start a worldwide pandemic. The virus's rapid evolution allows the virus to evade the host defense, and then seasonal vaccines need to be reformulated nearly annually. However, it takes almost half a year for the influenza vaccine to become accessible. This delay is especially concerning in the event of a pandemic breakout. By producing the vaccine through reverse vaccinology and phage display vaccines, this time can be reduced. In this study, epitopes of B lymphocytes, cytotoxic T lymphocytes, and helper T lymphocytes of HA, NA, NP, and M2 proteins from two strains of Influenza A were anticipated. We found two proper epitopes (ASFIYNGRL and LHLILWITDRLFFKC) in Influenza virus proteins for CTL and HTL cells, respectively. Optimal epitopes and linkers in silico were cloned into the N-terminal end of M13 protein III (pIII) to create a multi-epitope-pIII construct, i.e., phage display vaccine. Also, prediction of tertiary structure, molecular docking, molecular dynamics simulation, and immune simulation were performed and showed that the designed multi-epitope vaccine can bind to the receptors and stimulate the immune system response.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512060PMC
http://dx.doi.org/10.1038/s41598-024-74438-wDOI Listing

Publication Analysis

Top Keywords

influenza vaccine
8
phage display
8
influenza
5
vaccine
5
designing multi-epitope
4
multi-epitope influenza
4
vaccine immunoinformatics
4
immunoinformatics approach
4
approach influenza
4
influenza continues
4

Similar Publications

Background: The 2023-24 U.S. influenza season was characterized by a predominance of A(H1N1)pdm09 virus circulation with co-circulation of A(H3N2) and B/Victoria viruses.

View Article and Find Full Text PDF

Importance: Preterm infants are recommended to receive most vaccinations at the same postnatal age as term infants. Studies have inconsistently observed an increased risk for postvaccination apnea in preterm infants.

Objective: To compare the proportions of hospitalized preterm infants with apnea and other adverse events in the 48 hours after 2-month vaccinations vs after no vaccinations.

View Article and Find Full Text PDF

Respiratory syncytial virus (RSV) is the leading cause of acute lower respiratory infections in babies across the world. Irrespective of progress in the development of RSV vaccines, effective small molecule drugs are still not available on the market. Based on our previous data we designed and synthesized triazole-linked coumarin-monoterpene hybrids and showed that they are indeed effective in inhibiting the RSV replication.

View Article and Find Full Text PDF

Rabies is a zoonotic viral disease transmitted mainly by bites of infected animals, especially dogs, which are responsible for 99% of human cases. Despite being preventable, it remains a neglected disease in low-income countries, with approximately 60,000 deaths per year, mostly concentrated in Africa and Asia. The real worldwide burden of rabies is probably underestimated, as death-reporting systems are inadequate and active surveillance is limited.

View Article and Find Full Text PDF

Health is a precious asset, essential for both individuals and communities. The wars that have affected various parts of the world in recent years have had a detrimental impact on health, leading to malnutrition and an increased vulnerability to epidemic diseases among the population, especially the poorest. Hospitals and healthcare facilities themselves have become primary strategic targets in many war zones.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!