AI Article Synopsis
- - Citrus peels are emerging as a valuable type of biomass for biorefinery due to their low lignin and high sugar content, and this study found they can be processed enzymatically without pretreatment.
- - Researchers developed a specialized cellulase cocktail using response surface methodology (RSM) to enhance high-solids saccharification, achieving an optimized mixture and a substrate loading of 22%.
- - The study resulted in a high total sugar concentration of 123.84 g/L and an impressive sugar yield of 93.12%, highlighting the potential of citrus waste as a feedstock for producing sugars.
Article Abstract
Citrus peels, characterized by their low lignin and high sugar content, have been drawing increasing attention as a valuable lignocellulosic biomass with significant potential in biorefinery. Notably, in this study, the citrus waste was found to be enzymatically accessible without any pretreatment. Moreover, to promote the high-solids saccharification of the citrus peels, a tailored cellulase cocktail was formulated by response surface methodology (RSM), along with a fed-batch strategy aiming to obtain a high substrate loading. The study resulted in an optimized cellulase cocktail (7.08 U/g DM of β-glucosidase, 164.17 U/g DM of hemicellulase, 47.38 mg/g DM of sophorolipid, and 64.68 mg/g DM of Tween 80) and achieved solids loading of 22 % with a total sugar concentration of 123.84 g/L, corresponding to a yield of 93.12 % (65.28 % in batch operation). These findings provided essential validation for the efficient utilization of citrus waste, ensuring them promising potential as feedstock for sugar platforms.
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| http://dx.doi.org/10.1016/j.ijbiomac.2024.136863 | DOI Listing |
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