-3-Indoleacrylic Acid: A Nematicidal Compound from YMF3.862 as V-ATPase Inhibitor on .

The application of the bionematicides derived from microorganisms and their secondary metabolites represents a promising strategy for managing root-knot nematodes. In this study, a nematicidal compound, -3-indoleacrylic acid, was isolated from YMF3.862. This compound caused juveniles to have swollen bodies with apparent cracks on the cuticle surface. The LC value of -3-indoleacrylic acid against juveniles was 16.31 μg/mL 24 h of post-treatment. -3-indoleacrylic acid at 20 μg/mL significantly inhibited V-ATPase expression and remarkably decreased enzyme activity by 84.41%. As an inhibitor of V-ATPase, -3-indoleacrylic acid caused significant H accumulation in nematode bodies, resulting in lower intracellular pH values and higher extracellular pH values of . Application of 50 μg/mL -3-indoleacrylic acid generated a 71.06% control efficiency against on tomatoes. The combination results of this study indicated that -3-indoleacrylic acid can be developed as a natural nematicide for controlling .