AI Article Synopsis

  • The study investigated the causes of acute pneumonia in puppies in China by analyzing bronchoalveolar lavage (BAL) fluid to isolate and identify pathogens, as well as assess their antibiotic resistance and virulence genes.
  • Four extraintestinal pathogenic (ExPEC) strains were identified, showing resistance to certain antibiotics like cephalosporins and tetracyclines, but sensitivity to others such as aminoglycosides and carbapenems.
  • The research also found that the ExPEC strains were not present in the kennel’s drinking water, dismissing water contamination as a factor in the outbreak, and provided insights into potential treatments and mechanisms of the pathogens.

Article Abstract

To determine the etiological agents responsible for acute pneumonia in puppies in China, this study utilized bronchoalveolar lavage (BAL) fluid extraction to enable the isolation, culture, biochemical identification, and 16S rRNA PCR amplification of the pathogens. Following preliminary identification, the pathogens underwent analysis for antibiotic resistance phenotypes and resistance genes. Additionally, the study examined the presence of virulence genes, conducted multilocus sequence typing (MLST), and performed whole-genome sequencing (WGS). The findings revealed that all four isolated pathogens were characterized as extraintestinal pathogenic (ExPEC). The examined ExPEC strains demonstrated resistance to cephalosporins, tetracyclines, and penicillins, while remaining susceptible to aminoglycosides, beta-lactamase inhibitors, carbapenems, chloramphenicols, and sulfonamides. An analysis of virulence genes identified the presence of eight genes, namely , , , , , , , and , which are implicated in their invasiveness and potential to inflict tissue damage. The MLST analysis revealed that all ExPEC strains were classified under either sequence type ST131 (Achtman database) or ST43 (Pasteur database). The study further determined that these strains were absent in the kennel's drinking water source, thereby ruling out water contamination as a potential factor in the emergence of ST131-type ExPEC. This study offers a theoretical framework and empirical evidence for elucidating the potential pathogenic mechanisms and clinical therapeutic strategies of ExPEC in the etiology of acute pneumonia in puppies.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512281PMC
http://dx.doi.org/10.3390/vetsci11100491DOI Listing

Publication Analysis

Top Keywords

virulence genes
12
extraintestinal pathogenic
8
antibiotic resistance
8
sequence typing
8
acute pneumonia
8
pneumonia puppies
8
expec strains
8
genes
5
expec
5
characterization extraintestinal
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!