AI Article Synopsis
- The study investigates how oxalate affects rat renal tubular epithelial cells using a proteomic approach to identify changes in protein expression.
- It found 268 different proteins that were expressed differently in cells treated with oxalate compared to control cells, highlighting pathways like oxidative stress and inflammation.
- Certain proteins were identified as potential biomarkers and therapeutic targets, providing insights into preventing kidney damage and kidney stone formation caused by high oxalate levels.
Article Abstract
Our study aimed to apply a proteomic approach to investigate the molecular mechanisms underlying the effects of oxalate on rat renal tubular epithelial cells. NRK-52E cells were treated with or without oxalate and subjected to quantitative proteomics to identify key proteins and key pathological changes under high oxalate stimulation. A total of 268 differentially expressed proteins (DEPs) between oxalate-treated and control groups were identified, with 132 up-regulated and 136 down-regulated proteins. Functional enrichment analysis revealed that DEPs are associated with oxidative stress, apoptosis, ferroptosis, pro-inflammatory cytokines, vitamin D, and biomineralization. SPP1, MFGE8, ANKS1A, and NAP1L1 were up-regulated in the oxalate-treated cells and the hyperoxaluric stone-forming rats, while SUB1, RNPS1, and DGLUCY were down-regulated in both cases. This altered proteomic landscape sheds light on the pathological processes involved in oxalate-induced renal damage and identifies potential biomarkers and therapeutic targets to mitigate the effects of hyperoxaluria and reduce the risk of CaOx stone formation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505525 | PMC |
| http://dx.doi.org/10.3390/biology13100814 | DOI Listing |
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