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Efficient gene disruption in polyploid genome by Cas9-Trex2 fusion protein. | LitMetric

Efficient gene disruption in polyploid genome by Cas9-Trex2 fusion protein.

J Integr Plant Biol

State Key Laboratory of Wheat Improvement, Shandong Laboratory of Advanced Agricultural Sciences in Weifang, Peking University Institute of Advanced Agricultural Sciences, Weifang, 261325, China.

Published: October 2024

AI Article Synopsis

  • The combination of Trex2 exonuclease with Cas9 protein improved genome editing efficiency in hexaploid common wheat.
  • This fusion allows for the simultaneous editing of multiple desirable traits in just one generation.
  • It supports the advancement of genome editing-assisted breeding specifically in polyploid crops, making it easier for scientists to enhance crop varieties.

Article Abstract

The fusion of the exonuclease Trex2 with the Cas9 protein significantly enhanced the efficiency of genome editing in hexaploid common wheat, particularly for the simultaneous editing of multiple favorable alleles within a single generation, thereby facilitating genome editing-assisted breeding in polyploid crops.

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Source
http://dx.doi.org/10.1111/jipb.13797DOI Listing

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