Effects of water immersion on immune, intestinal flora and metabolome of Chinese mitten crab (Eriocheir sinensis) after air exposure.

Comp Biochem Physiol C Toxicol Pharmacol

Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Engineering Technology Research Center of Saline-alkaline Water Fisheries (Harbin), Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, People's Republic of China. Electronic address:

Published: January 2025

AI Article Synopsis

  • Air exposure causes significant stress in Chinese mitten crabs (Eriocheir sinensis), impacting their immune function and normal life activities, which can be alleviated by subsequent water immersion.* -
  • Immersion helps restore critical enzyme activities and reduces negative changes in glucose and cholesterol levels, with noticeable recovery seen within 24 hours after re-immersion.* -
  • Despite some recovery, air exposure still disrupts intestinal microbial diversity and metabolic balance, leading to persistent issues within the crabs' metabolism and nutrient absorption.*

Article Abstract

Air exposure stress can induce stress response of Eriocheir sinensis and affect its normal life activities. The goal of this study was to investigate the effects of water immersion on the recovery of hepatopancreas immune-related enzyme activity, intestinal microbial diversity and metabolic level of Chinese mitten crabs after exposure to air. The results show that immersion can effectively alleviate the adverse effects of air exposure on the antioxidant capacity and immune capacity of Chinese mitten crabs, and the longer the time of immersion, the more obvious the recovery effect. Among them, the levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and acid phosphatase significantly increased after exposure to air (P < 0.05), reached a peak at 3 h, began to decline after immersion, and returned to a level close to the initial value at 24 h (P < 0.05). In addition, after exposure to air, the glucose and total cholesterol in haemolymph of Eriocheir sinensis were significantly different from the initial values (P < 0.05), gradually recovered to the initial level after re-immersion. However, changes in intestinal flora and hepatopancreas metabolism caused by air exposure did not fully recover after water exposure, and its negative effects did not completely disappear. The sequencing results showed that the species composition and diversity of intestinal microorganisms of Chinese mitten crab changed after air exposure and immersion treatment. The relative abundance of Actinomycetes increased significantly, while that of Proteobacteria and Firmicutes decreased significantly. Metabolomics analysis showed that air exposure and immersion destroyed the metabolic balance of amino acids and carnitine, reduced the level of carnitine metabolism, hindered the absorption of nutrients, and led to the accumulation of harmful substances.

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Source
http://dx.doi.org/10.1016/j.cbpc.2024.110060DOI Listing

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