AI Article Synopsis
- The study utilized core genome single-nucleotide polymorphism (SNP) phylogeny to analyze a nosocomial outbreak of Vancomycin-resistant Enterococcus faecium (VREf), correctly identifying most related isolates as part of the outbreak cluster.
- Fourier-transform infrared (FT-IR) spectroscopy was effective in classifying the majority of these isolates and distinguishing unrelated samples, demonstrating its potential as a rapid screening tool for outbreak investigations.
- This is the inaugural research focusing on FT-IR spectroscopy's effectiveness in epidemiological analysis of VREf isolates sharing the same sequence type, marking a novel approach in microbiology.
Article Abstract
Background: Fourier-transform infrared (FT-IR) spectroscopy is a non-genomic, spectrum-based typing technology useful for characterizing outbreaks of multidrug-resistant organisms.
Aim: To assess the performance of FT-IR spectroscopy in characterizing ST-80 vancomycin-resistant Enterococcus faecium (VREf) isolates from a nosocomial outbreak.
Methods: Core-genome single-nucleotide polymorphism phylogeny was used as a reference method to characterize a nosocomial outbreak caused by ST-80 VREf. It identified 22 of 25 epidemiologically related isolates as belonging to an outbreak cluster.
Findings: The use of FT-IR spectroscopy with a cluster-defining cut-off of 0.071 resulted in the correct classification of 21 out of 22 phylogenetically related isolates in a single cluster. It successfully distinguished three phylogenetically unrelated isolates from the outbreak cluster, along with five ST-80-unrelated control isolates, and five isolates from a previous outbreak in May 2023, yielding only one mischaracterized environmental isolate.
Conclusion: These findings support the potential use of FT-IR spectroscopy as a rapid screening tool to assist outbreak investigations. Notably, this study is the first to focus on the performance of FT-IR spectroscopy in the epidemiological analysis of VREf isolates with the same sequence type.
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| http://dx.doi.org/10.1016/j.jhin.2024.10.005 | DOI Listing |
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