Immunogenicity analysis based on VP1 and VP2 proteins of bovine enterovirus.

Virology

Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, China. Electronic address:

Published: December 2024

AI Article Synopsis

  • - Bovine enterovirus (BEV) infections can lead to various symptoms in cattle, impacting their respiratory, gastrointestinal, and reproductive health, and causing significant economic losses in the bovine industry globally.
  • - The study utilized a method called reverse transcription-polymerase chain reaction to create a chimeric protein from BEV's VP1 and VP2 genes, which was then used to immunize mice and test for immune response.
  • - Results indicated that this immunization reduced virus shedding and tissue damage, improving overall health in the infected animals, which supports the potential for developing a vaccine against BEV.

Article Abstract

Bovine enterovirus (BEV) infection manifests as a spectrum of clinical signs affecting the respiratory, gastrointestinal, and reproductive systems in cattle. Outbreaks of this disease results in large economic losses to the bovine industry worldwide. Currently there are no efficacious vaccines and medicines to prevent BEV infection. In the present study, reverse transcription-polymerase chain reaction was used to amplify the VP1 and VP2 genes of BEV, enabling the synthesis of a chimeric recombinant protein which contained partial sequences from both proteins. Subsequently, the emulsified purified proteins with Freund's adjuvant were used for triple-fold immunization of 4-week-old Institute of Cancer Research (ICR) mice. The mice were subsequently subjected to a challenge assay which elicited an immune response that was characterized by elevated titers of BEV-specific neutralizing antibodies. Notably, the results showed that the purification of pET32a-VP1 and pET32a-VP2 proteins markedly curtailed virus excretion and mitigated the histopathological damage usually associated with BEV infections. These were observed in the small intestines, kidneys and brain in infected animals. It also alleviated clinical symptoms such as hypothermia and weight loss. In summary, this study offers a theoretical and practical basis for BEV vaccine development.

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Source
http://dx.doi.org/10.1016/j.virol.2024.110260DOI Listing

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