In aquaculture breeding or production programmes, counting juvenile fish represents a considerable cost in terms of the human hours needed. In this study, we explored the use of two state-of-the-art machine learning architectures (Single Shot Detection, hereafter SSD and Faster Regions with convolutional neural networks, hereafter Faster R-CNN) to augment a manual image-based juvenile fish counting method for the Australasian snapper () bred at The New Zealand Institute for Plant and Food Research Limited. We tested model accuracy after tuning for confidence thresholds and non-maximal suppression overlap parameters, and implementing a bias correction using a Poisson regression model. Validation of image data showed that after tuning, bias-corrected SSD and Faster R-CNN models had mean absolute percent errors (MAPE) of less than 10%, with SSD having MAPE of less than 5%. Comparison of the results with those from manual counts showed that, while manual counts are slightly more accurate (MAPE = 1.56), the machine learning methods allow for more rapid assessment of counts and thus facilitating a higher throughput. This work represents a first step for deploying machine learning applications to an existing real-life aquaculture scenario and provides a useful starting point for further developments, such as real-time counting of fish or collecting additional phenotypic data from the source images.
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http://dx.doi.org/10.1080/03036758.2022.2101484 | DOI Listing |
Metabolites
November 2024
Key Laboratory of Smart Agricultural Technology in the Southwest Mountains, Ministry of Agriculture and Rural Affairs (Co-Construction by Ministry and Province), Chongqing Academy of Agricultural Sciences, Chongqing 400715, China.
Ammonia, a ubiquitous contaminant in aquatic ecosystems, poses multifaceted threats to fish species at elevated concentrations. In order to investigate the toxic effects of chronic ammonia stress on the liver of juvenile , the present experiment was conducted to investigate the differences in changes in liver tissue structure, enzyme activities, and metabolomes after 28 days of ammonia exposure (0, 4, 8, and 16 mg/L). The findings revealed that ammonia exposure induced significant oxidative stress in the liver, manifesting in decreased activities of antioxidant enzymes SOD and GSH-Px, elevated levels of GSH, GST, and MDA, and heightened activities of immune enzymes LZM, ALP, and ACP.
View Article and Find Full Text PDFBioTech (Basel)
December 2024
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Fresh-Water Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
An eight-week trial was designed to study the effects of arginine (Arg) supplemented diets on the growth, immunity, antioxidant activity, and oxygen-carrying capacity of juvenile Gibel carp (). A total of 300 fish (27.53 ± 0.
View Article and Find Full Text PDFComp Biochem Physiol C Toxicol Pharmacol
December 2024
Edmonton, Alberta, Canada. Electronic address:
The effects of plastic pollution on marine organisms is of growing concern. The hydrophobic surface of plastics adsorbs organic contaminants and can alter the rate of chemical uptake in fishes. Per-fluorinated organic chemicals such as Perfluorooctanoic acid (PFOA) are highly hydrophobic toxic chemicals that adsorb to hydrophobic surfaces.
View Article and Find Full Text PDFFish Shellfish Immunol
December 2024
Norwegian Veterinary Institute, Postboks 64, 1431 Ås, Norway. Electronic address:
Infectious Salmon Anaemia virus (ISAV) is an orthomyxovirus that causes large economic losses in Atlantic salmon (Salmo salar L.) aquaculture. All virulent ISAV variants originally emerged from a non-virulent subtype, ISAV-HPR0.
View Article and Find Full Text PDFJ Therm Biol
December 2024
Department of Biology, Faculty of Natural Sciences, Norwegian University of Science and Technology, Trondheim, 7491, Norway; Department of Biology and Environmental Sciences, Kristineberg Center, University of Gothenburg, Fiskebäckskil, Sweden.
Climate warming with associated heat waves presents a concerning challenge for ectotherms such as fishes. During heatwaves, the ability to rapidly acclimate can be crucial for survival. However, surprisingly little is known about how different species and life stages vary in their acclimation dynamics, including the magnitude of change in thermal tolerance through acclimation (i.
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