Amoebic liver abscess (ALA) is a significant public health concern in tropical countries. Traditional diagnostic methods, such as microscopy, have low sensitivity, and nested polymerase chain reaction (nPCR) has lengthy turnaround times. In this study, we aimed to evaluate the effectiveness of the recombinase polymerase amplification (RPA) assay for detecting Entamoeba histolytica in amoebic liver pus samples. The assay was tested on 150 clinical pus samples collected from suspected ALA patients in the emergency department of a tertiary care center located in Chandigarh, northern India. For comparison with RPA, nPCR was also performed on these samples. Of 150 samples, 79 (53%) tested positive for E. histolytica using the RPA assay, and similar (79; 53%) number of samples tested positive with nested PCR. In addition, sequences obtained through Sanger sequencing showed high similarity with other sequences in the National Center for Biotechnology Information database and were submitted to the database. Our findings highlight the potential of RPA as a valuable tool for the accurate diagnosis of ALA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4269/ajtmh.24-0269 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A rare case of amebic liver abscess with spontaneous hepatico-cholecystic as well as cholecysto-duodenal fistula.
Med J Armed Forces India
December 2024
Senior Advisor (Medicine) & Gastroenterologist, Command Hospital, (Southern Command), Pune, India.
Massive gastrointestinal hemorrhage and iodine-125 seed migration due to hepatogastric fistula: a case report and literature review.
Transl Cancer Res
November 2024
Mini-invasive Intervention Center, The Third Affiliated Hospital of the Naval Medical University, Shanghai, China.
Background: Hepatogastric fistula (HGF) is an uncommon occurrence that can be associated with various medical conditions. The primary causes typically involve peptic ulcer disease, infections (such as pyogenic, amoebic or tuberculosis), or iatrogenic factors (like post transarterial chemoembolization or radiotherapy). Massive gastrointestinal hemorrhage following HGF is extremely rare, with iodine-125 (I) seed migration to the stomach through HGF not previously documented.
View Article and Find Full Text PDF-induced NETs are highly cytotoxic on hepatic and colonic cells due to serine proteases and myeloperoxidase activities.
Front Immunol
December 2024
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, Mexico.
During intestinal and liver invasion by the protozoan parasite , extensive tissue destruction linked to large neutrophil infiltrates is observed. It has been proposed that microbicidal components of neutrophils are responsible for the damage, however, the mechanism by which they are released and act in the extracellular space remains unknown. In previous studies, we have shown that trophozoites induce NET formation, leading to the release of neutrophil granule content into extruded DNA.
View Article and Find Full Text PDFDifficulty Confirming the Diagnosis of Amoebic Enteritis.
Tokai J Exp Clin Med
December 2024
Department of Gastroenterology, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
Article Synopsis
- Amoebic dysentery is a serious disease in Japan, classified as Category 5, with around 1,000 annual cases, and about 10% are invasive strains.
- Most invasive cases show no symptoms, making them hard to detect, especially since a key blood test was discontinued in 2017.
- Proper diagnosis and treatment are crucial to avoid severe complications, such as ulceration and liver abscesses, which can occur with delayed care.
Introduction Liver abscesses are one of the most concerning healthcare issues in Western countries, with a prevalence rate of three per 100,000. Although with the improvement in the socio-economic status and the health care system, its incidence has decreased in developed nations, pyogenic and amoebic liver abscesses are still high in resource-poor countries. Low socioeconomic conditions, improper hygiene, lack of awareness, and often a delay in diagnosis can lead to fatal complications and high mortality.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!