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A small-molecule carrier for the intracellular delivery of a membrane-impermeable protein with retained bioactivity. | LitMetric

AI Article Synopsis

  • Intracellular protein delivery has significant potential in cell biology and medicine, aiding in areas like bioimaging, disease treatment, and genome editing.* -
  • Researchers successfully delivered the functional protein cytochrome c (CYC) into lipid vesicles and live cells using a boron cluster anion carrier, achieving a cellular uptake rate of nearly 90%.* -
  • The method allowed CYC to enter the cytoplasm directly while maintaining its biological activity, showing a 25% increase in cell apoptosis at a low dose, highlighting the efficiency of inorganic cluster ions as protein delivery tools for future applications.*

Article Abstract

Intracellular protein delivery has the potential to revolutionize cell-biological research and medicinal therapy, with broad applications in bioimaging, disease treatment, and genome editing. Herein, we demonstrate successful delivery of a functional protein, cytochrome c (CYC), by using a boron cluster anion as molecular carrier of the superchaotropic anion type (BBrOPr). CYC was delivered into lipid bilayer vesicles as well as living cells, with a cellular uptake ratio approaching 90%. Mechanistic studies showed that CYC was internalized into cells through a permeation pathway directly into the cytoplasm, bypassing endosomal entrapment. Upon carrier-assisted internalization, CYC retained its bioactivity, as reflected by an induced cell apoptosis rate of 25% at low dose (1 µM). This study furbishes a direct protein delivery method by a molecular carrier with high efficiency, confirming the potential of inorganic cluster ions as protein transport vehicles with an extensive range of future cell-biological or biomedical applications.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536097PMC
http://dx.doi.org/10.1073/pnas.2407515121DOI Listing

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