Efficient enriching high-performance denitrifiers using bio-cathode of microbial fuel cells.

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Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi Province 712100, China.

Published: October 2024

Recent advancements in microbial fuel cells (MFC) technology have significantly contributed to the development of bio-cathode denitrification as a promising method for eco-friendly wastewater treatment. This study utilized an efficient repeated replacement method to enrich a mixed bio-cathode denitrifying culture (MBD) within a bio-cathode MFC, achieving a stable maximum output voltage of 120 ± 5 mV and a NO -N removal efficiency of 69.99 ± 0.60%. The electrotrophic denitrification process appears to be facilitated by electron shuttles. Microbial community analysis revealed a predominance of , with and as functional genera. Additionally, the isolated strain Lyy (belonging to ) from MBD demonstrated exceptional denitrification efficiencies exceeding 98% when treating wastewater with a broad range of C/N (2-12) ratios and KNO concentrations (500-3000 mg/L) within 60 h. These results demonstrated the effectiveness of the repeated replacement method in enriching bio-cathode denitrifiers and advancing MFC application in sustainable wastewater management.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492332PMC
http://dx.doi.org/10.1016/j.isci.2024.110965DOI Listing

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