Recent advancements in microbial fuel cells (MFC) technology have significantly contributed to the development of bio-cathode denitrification as a promising method for eco-friendly wastewater treatment. This study utilized an efficient repeated replacement method to enrich a mixed bio-cathode denitrifying culture (MBD) within a bio-cathode MFC, achieving a stable maximum output voltage of 120 ± 5 mV and a NO -N removal efficiency of 69.99 ± 0.60%. The electrotrophic denitrification process appears to be facilitated by electron shuttles. Microbial community analysis revealed a predominance of , with and as functional genera. Additionally, the isolated strain Lyy (belonging to ) from MBD demonstrated exceptional denitrification efficiencies exceeding 98% when treating wastewater with a broad range of C/N (2-12) ratios and KNO concentrations (500-3000 mg/L) within 60 h. These results demonstrated the effectiveness of the repeated replacement method in enriching bio-cathode denitrifiers and advancing MFC application in sustainable wastewater management.
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http://dx.doi.org/10.1016/j.isci.2024.110965 | DOI Listing |
Bioelectrochemistry
December 2024
School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353 Shandong, PR China; State Key Laboratory of Bio-based Materials and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, Shandong, PR China. Electronic address:
Sediment microbial fuel cell (SMFC) is a device for biological denitrification, in which electrons produced by sediment microorganisms can be transferred to the upper layer of the water column lacking electron donors. However, the low efficiency of denitrifying bacteria in acquiring electrons and enriching at the cathode greatly hinders the application of SMFC for nitrogen removal. In this study, we report a novel method of constructing a high-performance biocathode by modifying electrodes with zero-valent iron to enhance the enrichment and electron transfer of electroactive bacteria.
View Article and Find Full Text PDFBiosensors (Basel)
December 2024
Fujian Key Laboratory of Translational Research in Cancer and Neurodegenerative Diseases, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350108, China.
Simultaneous monitoring of antimicrobial responses to bacterial metabolic activity and biofilm formation is critical for efficient screening of new anti-biofilm drugs. A microbial fuel cell-based biosensor using as an electricigen was constructed. The effects of silver nanoparticles (AgNPs) on the cellular metabolic activity and biofilm formation of in the biosensors were investigated and compared with the traditional biofilm detection method.
View Article and Find Full Text PDFBiosensors (Basel)
November 2024
Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology, 02300 Vilnius, Lithuania.
Microbial fuel cells (MFCs) are a candidate for green energy sources due to microbes' ability to generate charge in their metabolic processes. The main problem in MFCs is slow charge transfer between microorganisms and electrodes. Several methods to improve charge transfer have been used until now: modification of microorganisms by conductive polymers, use of lipophilic mediators, and conductive nanomaterials.
View Article and Find Full Text PDFMicrobiol Resour Announc
December 2024
Marine Laboratory, Duke University, Beaufort, North Carolina, USA.
The microalga is an important organism for algae-based biocommodity production of food, feed, and fuel, among other products. Using PacBio Revio, we sequenced, assembled, and annotated a 26.41 Mbp C018 genome.
View Article and Find Full Text PDFMetab Eng Commun
December 2024
Instituto de Tecnologia Química e Biológica António Xavier, Oeiras, Portugal.
Polyethylene Terephthalate (PET) is a petroleum-based plastic polymer that, by design, can last decades, if not hundreds of years, when released into the environment through plastic waste leakage. In the pursuit of sustainable solutions to plastic waste recycling and repurposing, the enzymatic depolymerization of PET has emerged as a promising green alternative. However, the metabolic potential of the resulting PET breakdown molecules, such as the two-carbon (C2) molecule ethylene glycol (EG), remains largely untapped.
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