AI Article Synopsis

  • The AgNPs-Alg-Chi nanocomposite film shows strong antibacterial effects against pure bacterial strains and those from acne patients, but its cytotoxicity needs more exploration at higher silver doses.* -
  • The films were made using eco-friendly methods, featuring kumquat extract to reduce silver ions, and were characterized through various advanced techniques.* -
  • Results indicate that the films have comparable antibacterial potency to solutions of silver nanoparticles while maintaining over 70% cell viability in human keratinocytes, suggesting they are safe for potential human applications.*

Article Abstract

The silver nanoparticles-sodium alginate-chitosan (AgNPs-Alg-Chi) nanocomposite film is a compelling material with demonstrated antibacterial efficacy against various pure bacterial strains. However, its potential cytotoxicity at elevated Ag doses warrants investigation. There is a notable dearth of studies assessing its antibacterial effectiveness against clinically relevant bacterial strains, notably . This study aims to assess the antibacterial efficacy of the low-dose AgNPs-Alg-Chi nanocomposite films on both pure bacterial strains and strains isolated from clinical samples obtained from 65 acne patients. The films were synthesized using green methods, incorporating kumquat () extract as a silver ion-reducing agent. The material characterization methods include UV-Vis and FTIR spectroscopies, SEM-EDS, XPS, cell culture, and MTT assay. We successfully fabricated the AgNPs-Alg-Chi nanocomposite films with a low-loading dose of Ag NPs (≤11 μg mL, and 37.8 ± 11.5 nm in size). The AgNPs-Alg-Chi nanocomposite film demonstrated comparable antibacterial efficacy to the AgNPs-Chi solution, with MIC values ranging from 3.67 to 5.50 μg mL ( > 0.05) across all strains. Importantly, the AgNPs-Alg-Chi films demonstrated excellent biocompatibility with human keratinocytes (HaCaT cells), maintaining cell viability above 70%. The present AgNPs-Alg-Chi nanocomposite films synthesized by a green approach demonstrated potent antibacterial activity, making them promising for further development into suitable products for human use.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492429PMC
http://dx.doi.org/10.1039/d4ra05180gDOI Listing

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Article Synopsis
  • The AgNPs-Alg-Chi nanocomposite film shows strong antibacterial effects against pure bacterial strains and those from acne patients, but its cytotoxicity needs more exploration at higher silver doses.* -
  • The films were made using eco-friendly methods, featuring kumquat extract to reduce silver ions, and were characterized through various advanced techniques.* -
  • Results indicate that the films have comparable antibacterial potency to solutions of silver nanoparticles while maintaining over 70% cell viability in human keratinocytes, suggesting they are safe for potential human applications.*
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