AI Article Synopsis
- The text outlines a protocol for studying T cell activation using anti-CD3/CD28 stimulation, a common experimental method.
- It provides a workflow that includes isolating human peripheral blood mononuclear cells (PBMCs), enriching CD4 T cells, and performing single-cell RNA sequencing (scRNA-seq).
- For detailed procedures and execution of this protocol, the text refers to a more comprehensive source by Li et al.
Article Abstract
Stimulation of CD4 T cells with anti-CD3/CD28 is a commonly used model to study T cell activation. Here, we present a protocol for investigating T cell activation based on anti-CD3/CD28 bead stimulation and single-cell RNA sequencing (scRNA-seq). We describe the workflow from the isolation of human peripheral blood mononuclear cells (PMBCs) and CD4 T cell enrichment to anti-CD3/CD28 bead stimulation, scRNA-seq, and data analysis. For complete details on the use and execution of this protocol, please refer to Li et al..
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533540 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103409 | DOI Listing |
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