AI Article Synopsis
- CRISPR genome editing is being actively applied to schistosomes and other flukes to enhance research on their biology and disease mechanisms.
- CRISPR gene knockout studies have revealed that certain genes related to liver fluke growth are linked to disease progression.
- The review outlines current advancements in the field, including the potential for creating transgenic organisms, and suggests important questions for future research.
Article Abstract
CRISPR genome editing is actively used for schistosomes and other flukes. The ability to genetically manipulate these flatworms enables deeper investigation of their (patho)biological nature. CRISPR gene knockout (KO) demonstrated that a liver fluke growth mediator contributes to disease progression. Genome safe harbor sites have been predicted in Schistosoma mansoni and targeted for transgene insertion. CRISPR-based diagnosis has been demonstrated for infection with schistosomes and Opisthorchis viverrini. This review charts the progress, and the state of play, and posits salient questions for the field to address. Derivation of heritably transgenic loss-of-function or gain-of-function lines is the next milestone.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11560492 | PMC |
| http://dx.doi.org/10.1016/j.pt.2024.09.010 | DOI Listing |
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