A unique chitin-laccase membrane was fabricated as an environmentally friendly biocatalytic platform, utilizing 1-butyl-3-methylimidazolium acetate as the solvent for chitin. Observations using scanning electron microscopy showed that the chitin-laccase membrane possessed a uniform and densely packed structure. Based on the presence of FT-IR signals at 1020 cm and changes in the intensity of signals at 1540 cm and 1645 cm, the effectiveness of laccase immobilization was confirmed. FT-IR mapping revealed that the enzyme is evenly distributed on the surface of the membrane. The catalytic activity of the native enzyme and laccase immobilized using the membrane was determined based on a model reaction, and the retention of high activity was confirmed using real solutions. Laccase immobilized using the chitinous membrane retained over 60 % of its initial activity after 30 days of storage at 4 °C. By contrast, the free enzyme retained <40 % of its initial activity. Moreover, the activity of chitin-laccase system remained at 85 % after 5 cycles. This novel chitin-laccase combination was tested in the 17α-ethynylestradiol (EE2) removal from water-based solutions. It was found that EE2 underwent synergistic degradation through concurrent adsorption and biocatalytic transformation, with enzymatic conversion as the dominant mechanism.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2024.136599 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Laccase immobilized on reduced graphene oxide sponges for simultaneous adsorption and enzymatic degradation of endocrine disrupting chemicals.
Int J Biol Macromol
January 2025
Department of Chemical Engineering, Toronto Metropolitan University 350 Victoria Street, Toronto, Ontario M5B 2K3, Canada. Electronic address:
The presence of endocrine disrupting chemicals (EDCs) in water can impart detrimental effects on public health by mimicking the behaviors of natural hormones and their associated receptors in human body. Studies have demonstrated that ligninolytic enzymes such as laccase can degrade various phenolic compounds, including a broad range of EDCs. In this study, the technique of covalent immobilization of laccase through carbodiimide coupling chemistry on highly adsorptive reduced graphene oxide (rGO) sponges was utilized to effectively remove two representative EDCs; namely, bisphenol A (BPA) and triclosan (TCS) from water.
View Article and Find Full Text PDFUse of Laccase Enzymes as Bio-Receptors for the Organic Dye Methylene Blue in a Surface Plasmon Resonance Biosensor.
Sensors (Basel)
December 2024
Division de Fotónica, Centro de Investigaciones en Óptica AC, Loma del Bosque 115, Col. Lomas del Campestre, León 37150, Guanajuato, Mexico.
Methylene blue is a cationic organic dye commonly found in wastewater, groundwater, and surface water due to industrial discharge into the environment. This emerging pollutant is notably persistent and can pose risks to both human health and the environment. In this study, we developed a Surface Plasmon Resonance Biosensor employing a BK7 prism coated with 3 nm chromium and 50 nm of gold in the Kretschmann configuration, specifically for the detection of methylene blue.
View Article and Find Full Text PDFAdvancements in Biosensors for Lipid Peroxidation and Antioxidant Protection in Food: A Critical Review.
Antioxidants (Basel)
December 2024
Dipartimento di Chimica "Giacomo Ciamician", Alma Mater Studiorum-Università di Bologna, Via Gobetti 83, 40129 Bologna, Italy.
This review highlights the progress made in recent years on biosensors aimed at detecting relevant analytes/markers of food peroxidation. Starting from the basic definition of biosensors and the chemical features of peroxidation, here we describe the different approaches that can be used to obtain information about the progress of peroxidation and the efficacy of antioxidants. Aptamers, metal-organic frameworks, nanomaterials, and supported enzymes, in conjunction with electrochemical methods, can provide fast and cost-effective detection of analytes related to peroxidation, like peroxides, aldehydes, and metals.
View Article and Find Full Text PDFNano-structured polyaniline in biocatalysis: Manifesting simultaneous competence of polyaniline nanofibers and nanotubes as immobilization matrices for laccase mediated synthesis of drug intermediates.
Int J Biol Macromol
January 2025
Department of Pharmaceutical Technology, National Institute of Pharmaceutical Education and Research (NIPER) S.A.S. Nagar, Mohali, Punjab 160062, India. Electronic address:
Customized nano-biocatalysts of laccase have been made using nano-structured polyaniline viz. nano-fibers and nano-tubes, as immobilization supports and a simultaneous comparison between them has been made. Laccases are poly-phenol oxidases having tremendous utility concerning wider areas of application especially in the field of organic and drug syntheses.
View Article and Find Full Text PDFCharacterization and evaluation of the immobilized laccase enzyme potential in dye degradation via one factor and response surface methodology approaches.
Sci Rep
January 2025
Faculty of Biotechnology, German International University, Regional Ring Road, East Cairo, New Administrative Capital, Cairo, Egypt.
In the current study, calcium alginate was used as a carrier for Agaricus bisporus CU13 laccase immobilization, with an immobilization yield of the entrapped laccase of 91.95%. Free and immobilized enzymes showed their best enzyme activity at 60 °C as an optimum temperature.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!