The development of high-performance media for protein adsorption in bio-purification is highly desired, particularly in biological pharmaceuticals. In this study, we demonstrate a simple, versatile and mild strategy to construct a nanofibrous aerogel (NFA)-based adsorption media for protein purification. Pyromellitic dianhydride (PMDA) was selected to in-situ graft onto poly(ethylene-co-vinyl alcohol) (PE-co-PVA) nanofibers in aerogels through liquid phase grafting. The obtained PE-co-PVA/PMDA NFAs (PPNAs) possessed superb underwater elasticity, compression fatigue resistance and shape-memory performance. With an open porous network, abundant adsorption ligands, and surface hydrophilicity, the PPNAs exhibited a significant adsorption capacity of 1019.71 mg/g and a short equilibrium time of 3.0 h, surpassing that of commercial and reported nanofiber-based adsorbents. Additionally, the PPNAs demonstrated good dynamic adsorption performance for protein driven solely by gravity. Furthermore, the PPNAs showed reusability, selectivity, acid and alkaline resistance, and practical potential of extracting lysozyme form egg white solution. The successful scale-up of such aerogel-based adsorbents can open up new way for the development and design of next-generation protein adsorption media for bio-purification applications.
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| http://dx.doi.org/10.1016/j.ijbiomac.2024.136636 | DOI Listing |
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